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De Souza Gonçalves, L., Kratz, C., Santos, L. 
ORCID: 0000-0002-1915-6780, Henrique Carvalho, V., Peixoto Sales, L., Nemezio, K., Longobardi, I., Augusto Riani, L., Miranda de Oliveira Lima, M., Saito, T., Lins Fernandes, A., Rodrigues, J., James, R. ORCID: 0000-0002-7119-3159, Sale, C. ORCID: 0000-0002-5816-4169, Gualano, B., Geloneze, B., Helena Gennari de Medeiros, M. and Giannini Artioli, G.,
2020.
Insulin does not stimulate β-alanine transport into human skeletal muscle.
American Journal of Physiology - Cell Physiology, 318 (4), C777-C786.
ISSN 0363-6143
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Abstract
To test whether high circulating insulin concentrations influence the transport of β-alanine into skeletal muscle at either saturating or subsaturating β-alanine concentrations, we conducted two experiments whereby β-alanine and insulin concentrations were controlled. In experiment 1, 12 men received supraphysiological amounts of β-alanine intravenously (0.11 g·kg−1·min−1 for 150 min), with or without insulin infusion. β-Alanine and carnosine were measured in muscle before and 30 min after infusion. Blood samples were taken throughout the infusion protocol for plasma insulin and β-alanine analyses. β-Alanine content in 24-h urine was assessed. In experiment 2, six men ingested typical doses of β-alanine (10 mg/kg) before insulin infusion or no infusion. β-Alanine was assessed in muscle before and 120 min following ingestion. In experiment 1, no differences between conditions were shown for plasma β-alanine, muscle β-alanine, muscle carnosine and urinary β-alanine concentrations (all P > 0.05). In experiment 2, no differences between conditions were shown for plasma β-alanine or muscle β-alanine concentrations (all P > 0.05). Hyperinsulinemia did not increase β-alanine uptake by skeletal muscle cells, neither when substrate concentrations exceed the Vmax of β-alanine transporter TauT nor when it was below saturation. These results suggest that increasing insulin concentration is not necessary to maximize β-alanine transport into muscle following β-alanine intake.
| Item Type: | Journal article | ||||||||
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| Alternative Title: | Effects of insulin on β-alanine uptake by muscle | ||||||||
| Publication Title: | American Journal of Physiology - Cell Physiology | ||||||||
| Creators: | De Souza Gonçalves, L., Kratz, C., Santos, L., Henrique Carvalho, V., Peixoto Sales, L., Nemezio, K., Longobardi, I., Augusto Riani, L., Miranda de Oliveira Lima, M., Saito, T., Lins Fernandes, A., Rodrigues, J., James, R., Sale, C., Gualano, B., Geloneze, B., Helena Gennari de Medeiros, M. and Giannini Artioli, G. | ||||||||
| Publisher: | American Physiological Society | ||||||||
| Date: | April 2020 | ||||||||
| Volume: | 318 | ||||||||
| Number: | 4 | ||||||||
| ISSN: | 0363-6143 | ||||||||
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| Divisions: | Schools > School of Science and Technology | ||||||||
| Record created by: | Jonathan Gallacher | ||||||||
| Date Added: | 20 Mar 2020 13:17 | ||||||||
| Last Modified: | 31 May 2021 15:06 | ||||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/39422 |
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