Gene expression and functional comparison between multipotential stromal cells from lateral and medial condyles of knee osteoarthritis patients

Sanjurjo-Rodriguez, C., Baboolal, T.G., Burska, A.N., Ponchel, F., El-Jawhari, J.J. ORCID: 0000-0002-0580-4492, Pandit, H., McGonagle, D. and Jones, E., 2019. Gene expression and functional comparison between multipotential stromal cells from lateral and medial condyles of knee osteoarthritis patients. Scientific Reports, 9 (1): 9321.

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Abstract

Osteoarthritis (OA) is the most common degenerative joint disorder. Multipotential stromal cells (MSCs) have a crucial role in joint repair, but how OA severity affects their characteristics remains unknown. Knee OA provides a good model to study this, as osteochondral damage is commonly more severe in the medial weight-bearing compartment compared to lateral side of the joint. This study utilised in vitro functional assays, cell sorting, gene expression and immunohistochemistry to compare MSCs from medial and lateral OA femoral condyles. Despite greater cartilage loss and bone sclerosis in medial condyles, there was no significant differences in MSC numbers, growth rates or surface phenotype. Culture-expanded and freshly-purified medial-condyle MSCs expressed higher levels of several ossification-related genes. Using CD271-staining to identify MSCs, their presence and co-localisation with TRAP-positive chondroclasts was noted in the vascular channels breaching the osteochondral junction in lateral condyles. In medial condyles, MSCs were additionally found in small cavities within the sclerotic plate. These data indicate subchondral MSCs may be involved in OA progression by participating in cartilage destruction, calcification and sclerotic plate formation and that they remain abundant in severe disease. Biological or biomechanical modulation of these MSCs may be a new strategy towards cartilage and bone restoration in knee OA.

Item Type: Journal article
Publication Title: Scientific Reports
Creators: Sanjurjo-Rodriguez, C., Baboolal, T.G., Burska, A.N., Ponchel, F., El-Jawhari, J.J., Pandit, H., McGonagle, D. and Jones, E.
Publisher: Springer Science and Business Media LLC
Date: December 2019
Volume: 9
Number: 1
Identifiers:
NumberType
10.1038/s41598-019-45820-wDOI
1341699Other
Rights: © The Author(s) 2019. The Journal is open access by defualt. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Divisions: Schools > School of Science and Technology
Record created by: Linda Sullivan
Date Added: 07 Jul 2020 07:21
Last Modified: 07 Jul 2020 07:21
URI: http://irep.ntu.ac.uk/id/eprint/40178

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