Ancestral mitogenome capture of the Southeast Asian banded linsang

Paijmans, J.L.A., Barlow, A. ORCID: 0000-0002-5532-9458, Henneberger, K., Fickel, J., Hofreiter, M. and Foerster, D.W.G., 2020. Ancestral mitogenome capture of the Southeast Asian banded linsang. PLoS ONE, 15 (6): e0234385. ISSN 1932-6203

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Abstract

Utilising a reconstructed ancestral mitochondrial genome of a clade to design hybridisation capture baits can provide the opportunity for recovering mitochondrial sequences from all its descendent and even sister lineages. This approach is useful for taxa with no extant close relatives, as is often the case for rare or extinct species, and is a viable approach for the analysis of historical museum specimens. Asiatic linsangs (genus Prionodon) exemplify this situation, being rare Southeast Asian carnivores for which little molecular data is available. Using ancestral capture we recover partial mitochondrial genome sequences for seven banded linsangs (P. linsang) from historical specimens, representing the first intraspecific genetic dataset for this species. We additionally assemble a high quality mitogenome for the banded linsang using shotgun sequencing for time-calibrated phylogenetic analysis. This reveals a deep divergence between the two Asiatic linsang species (P. linsang, P. pardicolor), with an estimated divergence of ~12 million years (Ma). Although our sample size precludes any robust interpretation of the population structure of the banded linsang, we recover two distinct matrilines with an estimated tMRCA of ~1 Ma. Our results can be used as a basis for further investigation of the Asiatic linsangs, and further demonstrate the utility of ancestral capture for studying divergent taxa without close relatives.

Item Type: Journal article
Publication Title: PLoS ONE
Creators: Paijmans, J.L.A., Barlow, A., Henneberger, K., Fickel, J., Hofreiter, M. and Foerster, D.W.G.
Publisher: Public Library of Science
Date: 30 June 2020
Volume: 15
Number: 6
ISSN: 1932-6203
Identifiers:
NumberType
10.1371/journal.pone.0234385DOI
1341831Other
Rights: Copyright: © 2020 Paijmans et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Divisions: Schools > School of Science and Technology
Record created by: Jonathan Gallacher
Date Added: 07 Jul 2020 11:46
Last Modified: 13 Jul 2020 13:40
URI: http://irep.ntu.ac.uk/id/eprint/40182

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