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Novotny, E.N., White, S.J. 
ORCID: 0000-0002-3675-7545, Wilson, A.D., Stefánsdóttir, S.B., Tijhaar, E., Jonsdottir, S., Frey, R., Reiche, D., Rose, H., Rhyner, C., Schüpbach‐Regula, G., Torsteinsdottir, S., Alcocer, M. and Marti, E.,
2020.
Component‐resolved microarray analysis of IgE sensitization profiles to Culicoides recombinant allergens in horses with insect bite hypersensitivity.
Allergy.
ISSN 0105-4538
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1352315_a866_White.pdf - Published version Download (1MB) | Preview |
Abstract
Background: Allergy to bites of blood sucking insects, including biting midges can affect both human and veterinary patients. Horses are often suffering from an IgE‐mediated allergic dermatitis caused by bites of midges (Culicoides spp) . With the aim to improve allergen immunotherapy (AIT) numerous Culicoides allergens have been produced as recombinant (r‐) proteins. This study aims to test a comprehensive panel of differently expressed Culicoides r‐allergens on a cohort of IBH‐affected and control horses using an allergen microarray.
Methods: IgE levels to 27 Culicoides r‐allergens, including 8 previously unpublished allergens, of which 11 were expressed in more than one expression system, were determined in sera from 347 horses. ROC analyses were carried out, cut‐offs selected using a specificity of 95% and sero‐positivity rates compared between horses affected with insect bite hypersensitivity (IBH) and control horses. The combination of r‐allergens giving the best performing test was determined using logistic regression analysis.
Results: Sero‐positivity was significantly higher in IBH horses compared to controls for 25 r‐allergens. Nine Culicoides r‐allergens were major allergens for IBH with seven of them binding IgE in sera from >70% of the IBH‐affected horses. Combination of these top seven r‐allergens could diagnose >90% of IBH‐affected horses with a specificity of >95%. Correlation between differently expressed r‐allergens was usually high (mean = 0.69, range 0.28‐0.91).
Conclusion: This microarray will be a powerful tool for development of component‐resolved, patient‐tailored AIT for IBH and could be useful for the study of allergy to biting midges in humans and other species.
| Item Type: | Journal article | ||||||
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| Publication Title: | Allergy | ||||||
| Creators: | Novotny, E.N., White, S.J., Wilson, A.D., Stefánsdóttir, S.B., Tijhaar, E., Jonsdottir, S., Frey, R., Reiche, D., Rose, H., Rhyner, C., Schüpbach‐Regula, G., Torsteinsdottir, S., Alcocer, M. and Marti, E. | ||||||
| Publisher: | European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd. | ||||||
| Date: | 11 August 2020 | ||||||
| ISSN: | 0105-4538 | ||||||
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| Rights: | © 2020 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution‐Non Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. | ||||||
| Divisions: | Schools > School of Animal, Rural and Environmental Sciences | ||||||
| Record created by: | Linda Sullivan | ||||||
| Date Added: | 13 Aug 2020 09:36 | ||||||
| Last Modified: | 31 May 2021 15:17 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/40448 |
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