Analytical methods for the congener specific determination of polychlorinated biphenyls in environmental and food samples

Chewe, D., 1997. Analytical methods for the congener specific determination of polychlorinated biphenyls in environmental and food samples. PhD, Nottingham Trent University.

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Abstract

A method for the on-line extraction, clean-up, fractionation and congener specific determination of ortho- and non-ortho-PCBs by gas chromatography-mass spectrometry was developed and validated. The chromatographic clean-up and fractionation of PCBs was carried out on a silica multilayer column coupled to a supported carbon column. A solvent elution scheme for the efficient separation of ortho- and non-ortho-substituted polychlorinated biphenyls, in two different fractions was developed. Detection was by gas chromatography-mass spectrometry (GC-MS). The method was applied to the analysis of fruit and vegetable samples obtained from sites in South Wales including the vicinity of a chemical incinerator. In all cases where PCBs were detected, levels were <0.2 μg kg-1 Concentrations expressed in toxic equivalents (TEQs) showed that the most important contributor to the total TEQ was the non-ortho-PCB-126.

On-line high performance liquid chromatography-gas chromatography (HPLC-GC) is described for the concurrent determination of ortho- and non-ortho-substituted polychlorinated biphenyls (PCBs) in commercial mixture and soil samples. A porous graphitic carbon column eluted with hexane:toluene and toluene was used for the HPLC separation of ortho- and non-ortho-PCBs. Fractions were transferred from the HPLC to the GC column. Cryogenic cold trapping was used for analyte focusing at the head of the GC analytical column. Ortho- and non-ortho-PCB congeners were determined using electron capture and mass spectrometric detection.

Item Type:	Thesis
Creators:	Chewe, D.
Date:	1997
ISBN:	9781369313055
Identifiers:	Number Type PQ10183007 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Jeremy Silvester
Date Added:	28 Aug 2020 14:16
Last Modified:	14 Jun 2023 10:57
URI:	https://irep.ntu.ac.uk/id/eprint/40588

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