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Jones, W.G., El-Jawhari, J.J. 
ORCID: 0000-0002-0580-4492, Brockett, C.L., Koria, L., Ktistakis, I. and Jones, E.,
2021.
Multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – presence, potency and relationships to subchondral bone changes.
Journal of Cellular and Molecular Medicine, 25 (1), pp. 259-271.
ISSN 1582-1838
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Abstract
A large proportion of ankle osteoarthritis (OA) has an early onset and is post-traumatic. Surgical interventions have low patient satisfaction and relatively poor clinical outcome, whereas joint-preserving treatments, which rely on endogenous multipotential stromal cells (MSCs), result in suboptimal repair. This study investigates MSC presence and potency in OA-affected talocrural osteochondral tissue. Bone volume fraction (BV/TV) changes for the loading region trabecular volume and subchondral bone plate (SBP) thickness in OA compared with healthy tissue were investigated using microcomputed tomography. CD271-positive MSC topography was related to bone and cartilage damage in OA tissue, and in vitro MSC potency was compared with control healthy iliac crest (IC) MSCs. A 1.3- to 2.5-fold SBP thickening was found in both OA talus and tibia, whereas BV/TV changes were depth-dependent. MSCs were abundant in OA talus and tibia, with similar colony characteristics. Tibial and talar MSCs were tripotential, but talar MSCs had 10-fold lower adipogenesis and twofold higher chondrogenesis than IC MSCs (P = .01 for both). Cartilage damage in both OA tibia and talus correlated with SBP thickening and CD271+ MSCs was 1.4- to twofold more concentrated near the SBP. This work shows multipotential MSCs are present in OA talocrural subchondral bone, with their topography suggesting ongoing involvement in SBP thickening. Potentially, biomechanical stimulation could augment the chondrogenic differentiation of MSCs for joint-preserving treatments.
| Item Type: | Journal article | ||||||
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| Publication Title: | Journal of Cellular and Molecular Medicine | ||||||
| Creators: | Jones, W.G., El-Jawhari, J.J., Brockett, C.L., Koria, L., Ktistakis, I. and Jones, E. | ||||||
| Publisher: | Wiley | ||||||
| Date: | January 2021 | ||||||
| Volume: | 25 | ||||||
| Number: | 1 | ||||||
| ISSN: | 1582-1838 | ||||||
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| Rights: | © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | ||||||
| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Laura Ward | ||||||
| Date Added: | 28 May 2021 15:06 | ||||||
| Last Modified: | 31 May 2021 15:02 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/42953 |
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