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Chemmarappally, J.M., Pegram, H.C.N., Abeywickrama, N., Fornari, E., Hargreaves, A.J. 
ORCID: 0000-0001-9754-5477, De Girolamo, L.A. and Stevens, B. ORCID: 0000-0002-3270-4040,
2020.
A co-culture nanofibre scaffold model of neural cell degeneration in relevance to Parkinson’s disease.
Scientific Reports, 10: 2767.
ISSN 2045-2322
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Abstract
Current therapeutic strategies for Parkinson’s disease (PD) aim to delay progression or replace damaged neurons by restoring the original neuronal structures. The poor regenerative capacity of neural tissue highlights the need for the development of cellular environments to model the pathogenesis of PD. In the current work, we have characterised the growth, survival and response to PD mimetics of human SH-SY5Y neuroblastoma and U-87MG glioblastoma cell lines cultured on polyacrylonitrile (PAN) and Jeffamine® doped polyacrylonitrile (PJ) nano-scaffolds. Differentiation induced by a range of agents was evaluated by immunoassays of neural protein biomarkers. PAN and PJ nanofibre scaffolds provided suitable three-dimensional (3D) environment to support the growth, differentiation and network formation of dopaminergic neuron- and astrocyte-like cell populations, respectively. The scaffolds selectively supported the survival and differentiation of both cell populations with prolonged neuronal survival when exposed to PD mimetics in the presence of astrocytes in a co-culture model. Such 3D nanoscaffold-based assays could aid our understanding of the molecular basis of PD mimetic-induced Parkinsonism and the discovery of neuroprotective agents.
| Item Type: | Journal article | ||||||
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| Publication Title: | Scientific Reports | ||||||
| Creators: | Chemmarappally, J.M., Pegram, H.C.N., Abeywickrama, N., Fornari, E., Hargreaves, A.J., De Girolamo, L.A. and Stevens, B. | ||||||
| Publisher: | Springer | ||||||
| Date: | 17 February 2020 | ||||||
| Volume: | 10 | ||||||
| ISSN: | 2045-2322 | ||||||
| Identifiers: |
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| Rights: | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | ||||||
| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Laura Ward | ||||||
| Date Added: | 04 Jun 2021 13:23 | ||||||
| Last Modified: | 04 Jun 2021 13:54 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/42978 |
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