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Taylor, C.S., Chen, R., D' Sa, R., Hunt, J.A. 
ORCID: 0000-0002-5168-4778, Curran, J.M. and Haycock, J.W.,
2021.
Cost effective optimised synthetic surface modification strategies for enhanced control of neuronal cell differentiation and supporting neuronal and Schwann cell viability.
Journal of Biomedical Materials Research Part B: Applied Biomaterials.
ISSN 1552-4973
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1427005_Hunt.pdf - Published version Download (10MB) | Preview |
Abstract
Enriching a biomaterial surface with specific chemical groups has previously been considered for producing surfaces that influence cell response. Silane layer deposition has previously been shown to control mesenchymal stem cell adhesion and differentiation. However, it has not been used to investigate neuronal or Schwann cell responses in vitro to date. We report on the deposition of aminosilane groups for peripheral neurons and Schwann cells studying two chain lengths: (a) 3-aminopropyl triethoxysilane (short chain-SC) and (b) 11-aminoundecyltriethoxysilane (long chain-LC) by coating glass substrates. Surfaces were characterised by water contact angle, AFM and XPS. LC–NH2 was produced reproducibly as a homogenous surface with controlled nanotopography. Primary neuron and NG108-15 neuronal cell differentiation and primary Schwann cell responses were investigated in vitro by S100β, p75, and GFAP antigen expression. Both amine silane surface supported neuronal and Schwann cell growth; however, neuronal differentiation was greater on LC aminosilanes versus SC. Thus, we report that silane surfaces with an optimal chain length may have potential in peripheral nerve repair for the modification and improvement of nerve guidance devices.
| Item Type: | Journal article | ||||||
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| Publication Title: | Journal of Biomedical Materials Research Part B: Applied Biomaterials | ||||||
| Creators: | Taylor, C.S., Chen, R., D' Sa, R., Hunt, J.A., Curran, J.M. and Haycock, J.W. | ||||||
| Publisher: | Wiley | ||||||
| Date: | 21 March 2021 | ||||||
| ISSN: | 1552-4973 | ||||||
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| Rights: | © 2021 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | ||||||
| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Linda Sullivan | ||||||
| Date Added: | 15 Jul 2021 13:18 | ||||||
| Last Modified: | 15 Jul 2021 13:19 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/43484 |
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