Nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity

Cartwright, D.M., Oakey, L.A., Fletcher, R.S., Doig, C.L. ORCID: 0000-0001-9694-4230, Heising, S., Larner, D.P., Nasteska, D., Berry, C.E., Heaselgrave, S.R., Ludwig, C., Hodson, D.J., Lavery, G.G. ORCID: 0000-0001-5794-748X and Garten, A., 2021. Nicotinamide riboside has minimal impact on energy metabolism in mouse models of mild obesity. Journal of Endocrinology, 251 (1), pp. 111-123. ISSN 0022-0795

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Abstract

Supplementation with precursors of NAD has been shown to prevent and reverse insulin resistance, mitochondrial dysfunction, and liver damage in mouse models of diet-induced obesity. We asked whether the beneficial effects of supplementation with the NAD precursor nicotinamide riboside (NR) are dependent on mouse strain. We compared the effects of NR supplementation on whole-body energy metabolism and mitochondrial function in mildly obese C57BL/6N and C57BL/6J mice, two commonly used strains to investigate metabolism. Male C57BL/6N and C57BL/6J mice were fed a high-fat diet (HFD) or standard chow with or without NR supplementation for 8 weeks. Body and organ weights, glucose tolerance, and metabolic parameters as well as mitochondrial O2 flux in liver and muscle fibers were assessed. We found that NR supplementation had no influence on body or organ weight, glucose metabolism or hepatic lipid accumulation, energy expenditure, or metabolic flexibility but increased mitochondrial respiration in soleus muscle in both mouse strains. Strain-dependent differences were detected for body and fat depot weight, fasting blood glucose, hepatic lipid accumulation, and energy expenditure. We conclude that, in mild obesity, NR supplementation does not alter metabolic phenotype in two commonly used laboratory mouse strains.

Item Type:	Journal article
Publication Title:	Journal of Endocrinology
Creators:	Cartwright, D.M., Oakey, L.A., Fletcher, R.S., Doig, C.L., Heising, S., Larner, D.P., Nasteska, D., Berry, C.E., Heaselgrave, S.R., Ludwig, C., Hodson, D.J., Lavery, G.G. and Garten, A.
Publisher:	Bioscientifica
Date:	1 October 2021
Volume:	251
Number:	1
ISSN:	0022-0795
Identifiers:	Number Type 10.1530/joe-21-0123 DOI 1484864 Other
Rights:	© 2021 The authors. Published by Bioscientifica Ltd. This work is licensed under a Creative Commons Attribution 4.0 International License.
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	04 Nov 2021 10:09
Last Modified:	25 Mar 2022 11:53
URI:	https://irep.ntu.ac.uk/id/eprint/44610

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