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Surani, A.A. 
ORCID: 0000-0002-5180-9075 and Montiel-Duarte, C. ORCID: 0000-0002-9144-8809,
2022.
Native RNA G quadruplex Immunoprecipitation (rG4IP) from mammalian cells.
STAR Protocols, 3 (2): 101372.
ISSN 2666-1667
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Abstract
G quadruplex structures play an important role in regulating DNA replication and transcription and mRNA translation. Although there are several techniques that can determine its formation in vitro, the study of RNA G quadruplexes in vivo is not simple. In the current protocol, we describe an optimized technique (RNA G quadruplex immunoprecipitation [rG4IP]) to selectively pull down native cytoplasmic RNAs containing G quadruplex structures in mammalian cells. We also use a bicistronic plasmid to confirm and pinpoint the structure location.
For complete details on the use and execution of this protocol, please refer to Surani et al. (2022).
| Item Type: | Journal article | ||||||
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| Publication Title: | STAR Protocols | ||||||
| Creators: | Surani, A.A. and Montiel-Duarte, C. | ||||||
| Publisher: | Elsevier (Cell Press) | ||||||
| Date: | 17 June 2022 | ||||||
| Volume: | 3 | ||||||
| Number: | 2 | ||||||
| ISSN: | 2666-1667 | ||||||
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| Rights: | ©2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | ||||||
| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Laura Ward | ||||||
| Date Added: | 30 May 2022 08:26 | ||||||
| Last Modified: | 04 Jul 2022 15:29 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/46382 |
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