Native RNA G quadruplex Immunoprecipitation (rG4IP) from mammalian cells

Surani, A.A. ORCID: 0000-0002-5180-9075 and Montiel-Duarte, C. ORCID: 0000-0002-9144-8809, 2022. Native RNA G quadruplex Immunoprecipitation (rG4IP) from mammalian cells. STAR Protocols, 3 (2): 101372. ISSN 2666-1667

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Abstract

G quadruplex structures play an important role in regulating DNA replication and transcription and mRNA translation. Although there are several techniques that can determine its formation in vitro, the study of RNA G quadruplexes in vivo is not simple. In the current protocol, we describe an optimized technique (RNA G quadruplex immunoprecipitation [rG4IP]) to selectively pull down native cytoplasmic RNAs containing G quadruplex structures in mammalian cells. We also use a bicistronic plasmid to confirm and pinpoint the structure location.

For complete details on the use and execution of this protocol, please refer to Surani et al. (2022).

Item Type: Journal article
Publication Title: STAR Protocols
Creators: Surani, A.A. and Montiel-Duarte, C.
Publisher: Elsevier (Cell Press)
Date: 17 June 2022
Volume: 3
Number: 2
ISSN: 2666-1667
Identifiers:
NumberType
10.1016/j.xpro.2022.101372DOI
1539049Other
Rights: ©2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Divisions: Schools > School of Science and Technology
Record created by: Laura Ward
Date Added: 30 May 2022 08:26
Last Modified: 04 Jul 2022 15:29
URI: http://irep.ntu.ac.uk/id/eprint/46382

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