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Pickup, M.E. 
ORCID: 0000-0002-6006-2540, Hu, A. ORCID: 0000-0001-8821-6591, Patel, H.J. ORCID: 0000-0002-8461-9980 and Ahmed, M.I. ORCID: 0000-0002-9051-7681,
2022.
MicroRNA-148a controls epidermal and hair follicle stem/progenitor cells by modulating the activities of Rock1 and Elf5.
Journal of Investigative Dermatology.
ISSN 0022-202X
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Abstract
Skin and hair development is regulated by complex programs of gene activation and silencing and microRNA-dependent modulation of gene expression to maintain normal skin and hair follicle development, homeostasis, and cycling. In this study, we show that miR-148a, through its gene targets, plays an important role in regulating skin homeostasis and hair follicle cycling. RNA and protein analysis of miR-148a and its gene targets were analyzed using a combination of in vitro and in vivo experiments. We show that the expression of miR-148a markedly increases during telogen (bulge and hair germ stem cell compartments). Administration of antisense miR-148a inhibitor into mouse skin during the telogen phases of the postnatal hair cycle results in accelerated anagen development and altered stem cell activity in the skin. We also show that miR-148a can regulate colony-forming abilities of hair follicle bulge stem cells as well as control keratinocyte proliferation/differentiation processes. RNA and protein analysis revealed that miR-148a may control these processes by regulating the expression of Rock1 and Elf5 in vitro and in vivo. These data provide an important foundation for further analyses of miR-148a as a crucial regulator of these genes target in the skin and hair follicles and its importance in maintaining stem/progenitor cell functions during normal tissue homeostasis and regeneration.
| Item Type: | Journal article | ||||||
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| Publication Title: | Journal of Investigative Dermatology | ||||||
| Creators: | Pickup, M.E., Hu, A., Patel, H.J. and Ahmed, M.I. | ||||||
| Publisher: | Elsevier BV | ||||||
| Date: | 15 September 2022 | ||||||
| ISSN: | 0022-202X | ||||||
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| Rights: | © 2022 the authors. Published by Elsevier, Inc. on behalf of the Society for Investigative Dermatology. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | ||||||
| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Jonathan Gallacher | ||||||
| Date Added: | 03 Jan 2023 11:55 | ||||||
| Last Modified: | 03 Jan 2023 11:55 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/47707 |
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