Tools
Tools
Tarlinton, R.E., Legione, A.R., Sarker, N., Fabijan, J., Meers, J., McMichael, L., Simmons, G., Owen, H., Seddon, J.M., Dick, G., Ryder, J.S., Hemmatzedah, F., Trott, D.J., Speight, N., Holmes, N., Loose, M. and Emes, R.D. 
ORCID: 0000-0001-6855-5481,
2022.
Differential and defective transcription of koala retrovirus indicates the complexity of host and virus evolution.
Journal of General Virology, 103 (6): 001749.
ISSN 0022-1317
Abstract
Koala retrovirus (KoRV) is unique amongst endogenous (inherited) retroviruses in that its incorporation to the host genome is still active, providing an opportunity to study what drives this fundamental process in vertebrate genome evolution. Animals in the southern part of the natural range of koalas were previously thought to be either virus-free or to have only exogenous variants of KoRV with low rates of KoRV-induced disease. In contrast, animals in the northern part of their range universally have both endogenous and exogenous KoRV with very high rates of KoRV-induced disease such as lymphoma. In this study we use a combination of sequencing technologies, Illumina RNA sequencing of ‘southern’ (south Australian) and ‘northern’ (SE QLD) koalas and CRISPR enrichment and nanopore sequencing of DNA of ‘southern’ (South Australian and Victorian animals) to retrieve full-length loci and intregration sites of KoRV variants. We demonstrate that koalas that tested negative to the KoRV pol gene qPCR, used to detect replication-competent KoRV, are not in fact KoRV-free but harbour defective, presumably endogenous, ‘RecKoRV’ variants that are not fixed between animals. This indicates that these populations have historically been exposed to KoRV and raises questions as to whether these variants have arisen by chance or whether they provide a protective effect from the infectious forms of KoRV. This latter explanation would offer the intriguing prospect of being able to monitor and selectively breed for disease resistance to protect the wild koala population from KoRV-induced disease.
| Item Type: | Journal article | ||||||
|---|---|---|---|---|---|---|---|
| Publication Title: | Journal of General Virology | ||||||
| Creators: | Tarlinton, R.E., Legione, A.R., Sarker, N., Fabijan, J., Meers, J., McMichael, L., Simmons, G., Owen, H., Seddon, J.M., Dick, G., Ryder, J.S., Hemmatzedah, F., Trott, D.J., Speight, N., Holmes, N., Loose, M. and Emes, R.D. | ||||||
| Publisher: | Microbiology Society | ||||||
| Date: | 28 June 2022 | ||||||
| Volume: | 103 | ||||||
| Number: | 6 | ||||||
| ISSN: | 0022-1317 | ||||||
| Identifiers: |
|
||||||
| Rights: | This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution. | ||||||
| Divisions: | Professional Services > The Directorate | ||||||
| Record created by: | Jonathan Gallacher | ||||||
| Date Added: | 07 Feb 2023 16:01 | ||||||
| Last Modified: | 07 Feb 2023 16:01 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/48188 |
Actions (login required)
 |
Edit View |
Views
Views per month over past year
Downloads
Downloads per month over past year
