The role of microRNAs in skin development

Pickup, M.E. ORCID: 0000-0002-6006-2540, 2022. The role of microRNAs in skin development. PhD, Nottingham Trent University.

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Abstract

Skin development and hair follicle (HF) cycling are governed by diverse programs of gene modulation through activation and silencing. Post-transcriptional gene expression modulation by microRNAs are another regulatory mechanism to maintain normal skin and HF development and cycling. Here, we identify that miR-148a plays an important role in the regulation of skin homeostasis and HF cycling. RNA and protein analysis of miR-148a and its targets were analysed using both in vitro and in vivo experiments. Spatiotemporal expression analysis of miR-148a during hair cycling revealed an increase in miR-148a expression during the telogen stage, in the bulge and hair germ stem/progenitor cell compartments, and the differentiated, suprabasal layers of the epidermis. We demonstrate that miR-148a can control proliferation and differentiation programs in primary mouse epidermal keratinocytes. Administration of anti-sense miR-148a inhibitor into dorsal mouse skin during telogen stage of HF cycling accelerated telogen-anagen transition and altered stem cell activity, in vitro and in vivo. RNA and protein analysis revealed that miR-148a may control these cellular processes, in part, through modulation of Rock1 and Elf5. This data identifies a previously unknown role of miR-148a and novel gene targets in the control of skin and HF homeostasis and modulating stem/progenitor cell populations. This provides a potential target for stem cell biology research and therapeutic targets for the treatment of skin disorders.

Item Type:	Thesis
Creators:	Pickup, M.E.
Date:	July 2022
Rights:	The copyright in this work is held by the author. You may copy up to 5% of this work for private study, or personal, non-commercial research. Any re-use of the information contained within this document should be fully referenced, quoting the author, title, university, degree level and pagination. Queries or requests for any other use, or if a more substantial copy is required, should be directed to the author.
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	09 Aug 2023 09:27
Last Modified:	09 Aug 2023 09:33
URI:	https://irep.ntu.ac.uk/id/eprint/49531

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