Staphylococcus aureus lipase 1 enhances influenza A virus replication

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Goncheva, M.I., Conceicao, C., Tuffs, S.W., Lee, H.M., Quigg-Nicol, M., Bennet, I., Sargison, F., Pickering, A.C., Hussain, S., Gill, A.C. ORCID: 0000-0001-5201-9473, Dutia, B.M., Digard, P. and Fitzgerald, J.R., 2020. Staphylococcus aureus lipase 1 enhances influenza A virus replication. mBio, 11 (4): e00975-20. ISSN 2150-7511

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Official URL: https://doi.org/10.1128/mbio.00975-20

Abstract

Influenza A virus (IAV) causes annual epidemics of respiratory disease in humans, often complicated by secondary coinfection with bacterial pathogens such as Staphylococcus aureus. Here, we report that the S. aureus secreted protein lipase 1 enhances IAV replication in vitro in primary cells, including human lung fibroblasts. The proviral activity of lipase 1 is dependent on its enzymatic function, acts late in the viral life cycle, and results in increased infectivity through positive modulation of virus budding. Furthermore, the proviral effect of lipase 1 on IAV is exhibited during in vivo infection of embryonated hen’s eggs and, importantly, increases the yield of a vaccine strain of IAV by approximately 5-fold. Thus, we have identified the first S. aureus protein to enhance IAV replication, suggesting a potential role in coinfection. Importantly, this activity may be harnessed to address global shortages of influenza vaccines.

Item Type:

Journal article

Publication Title:

mBio

Creators:

Goncheva, M.I., Conceicao, C., Tuffs, S.W., Lee, H.M., Quigg-Nicol, M., Bennet, I., Sargison, F., Pickering, A.C., Hussain, S., Gill, A.C., Dutia, B.M., Digard, P. and Fitzgerald, J.R.

Publisher:

American Society for Microbiology

Date:

August 2020

Volume:

Number:

ISSN:

2150-7511

Identifiers: