Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species

KUCEROVA, E., CLIFTON, S.W., XIA, X.Q., LONG, F., PORWOLLIK, S., FULTON, L., FRONICK, C., MINX, P., KYUNG, K., WARREN, W., FULTON, R., FENG, D., WOLLAM, A., SHAH, N., BHONAGIRI, V., NASH, W.E., HALLSWORTH-PEPIN, K., WILSON, R.K., MCCLELLAND, M. and FORSYTHE, S.J., 2010. Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species. Plos One, 5 (3). ISSN 1932-6203

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Background: The genus Cronobacter (formerly called Enterobacter sakazakii) is composed of five species; C. sakazakii, C. malonaticus, C. turicensis, C. muytjensii, and C. dublinensis. The genus includes opportunistic human pathogens, and the first three species have been associated with neonatal infections. The most severe diseases are caused in neonates and include fatal necrotizing enterocolitis and meningitis. The genetic basis of the diversity within the genus is unknown, and few virulence traits have been identified. Methodology/Principal Findings: We report here the first sequence of a member of this genus, C. sakazakii strain BAA-894. The genome of Cronobacter sakazakii strain BAA-894 comprises a 4.4 Mb chromosome (57% GC content) and two plasmids; 31 kb (51% GC) and 131 kb (56% GC). The genome was used to construct a 387,000 probe oligonucleotide tiling DNA microarray covering the whole genome. Comparative genomic hybridization (CGH) was undertaken on five other C. sakazakii strains, and representatives of the four other Cronobacter species. Among 4,382 annotated genes inspected in this study, about 55% of genes were common to all C. sakazakii strains and 43% were common to all Cronobacter strains, with 10–17% absence of genes.

Item Type: Journal article
Publication Title: Plos One
Creators: Kucerova, E., Clifton, S.W., Xia, X.Q., Long, F., Porwollik, S., Fulton, L., Fronick, C., Minx, P., Kyung, K., Warren, W., Fulton, R., Feng, D., Wollam, A., Shah, N., Bhonagiri, V., Nash, W.E., Hallsworth-Pepin, K., Wilson, R.K., McClelland, M. and Forsythe, S.J.
Publisher: Public Library of Science
Date: 2010
Volume: 5
Number: 3
ISSN: 1932-6203
Rights: © 2010 Kucerova et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CCAL), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Divisions: Schools > School of Science and Technology
Depositing User: EPrints Services
Date Added: 09 Oct 2015 09:56
Last Modified: 09 Jun 2017 13:14

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