Lawton, M., Iqbal, M., Kontovraki, M., Lloyd Mills, C. and Hargreaves, A.J. ORCID: 0000-0001-9754-5477, 2007. Reduced tubulin tyrosination as an early marker of mercury toxicity in differentiating N2a cells. Toxicology in Vitro, 21 (7), pp. 1258-1261. ISSN 0887-2333
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Abstract
The aims of this work were to compare the effects of methyl mercury chloride and Thimerosal on neurite/process outgrowth and microtubule proteins in differentiating mouse N2a neuroblastoma and rat C6 glioma cells. Exposure for 4 h to sublethal concentrations of both compounds inhibited neurite outgrowth to a similar extent in both cells lines compared to controls. In the case of N2a cells, this inhibitory effect by both compounds was associated with a fall in the reactivity of western blots of cell extracts with monoclonal antibody T1A2, which recognises C-terminally tyrosinated α-tubulin. By contrast, reactivity with monoclonal antibody B512 (which recognises total α-tubulin) was unaffected at the same time point. These findings suggest that decreased tubulin tyrosination represents a neuron-specific early marker of mercury toxicity associated with impaired neurite outgrowth.
Item Type: | Journal article | ||||
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Publication Title: | Toxicology in Vitro | ||||
Creators: | Lawton, M., Iqbal, M., Kontovraki, M., Lloyd Mills, C. and Hargreaves, A.J. | ||||
Publisher: | Elsevier | ||||
Date: | 2007 | ||||
Volume: | 21 | ||||
Number: | 7 | ||||
ISSN: | 0887-2333 | ||||
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Rights: | Copyright © 2007 Elsevier Ltd All rights reserved. | ||||
Divisions: | Schools > School of Science and Technology | ||||
Record created by: | EPrints Services | ||||
Date Added: | 09 Oct 2015 10:33 | ||||
Last Modified: | 09 Jun 2017 13:33 | ||||
URI: | https://irep.ntu.ac.uk/id/eprint/14732 |
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