Barnes, I.H.A., Bagnall, M.C., Browning, D.D., Thompson, S.A., Manning, G. and Newell, D.G., 2007. γ-Glutamyl transpeptidase has a role in the persistent colonization of the avian gut by Campylobacter jejuni. Microbial Pathogenesis, 43 (56), pp. 198-207.
|
Text
197223_1941 Manning PostPrint.pdf Download (980kB) | Preview |
Abstract
The contribution of γ-glutamyl transpeptidase (GGT) to Campylobacter jejuni virulence and colonization of the avian gut has been investigated. The presence of the ggt gene in C. jejuni strains directly correlated with the expression of GGT activity as measured by cleavage and transfer of the γ-glutamyl moiety. Inactivation of the monocistronic ggt gene in C. jejuni strain 81116 resulted in isogenic mutants with undetectable GGT activity; nevertheless, these mutants grew normally in vitro. However, the mutants had increased motility, a 5.4-fold higher invasion efficiency into INT407 cells in vitro and increased resistance to hydrogen peroxide stress. Moreover, the apoptosis-inducing activity of the ggt mutant was significantly lower than that of the parental strain. In vivo studies showed that, although GGT activity was not required for initial colonization of 1-day-old chicks, the enzyme was required for persistant colonization of the avian gut.
Item Type: | Journal article |
---|---|
Publication Title: | Microbial Pathogenesis |
Creators: | Barnes, I.H.A., Bagnall, M.C., Browning, D.D., Thompson, S.A., Manning, G. and Newell, D.G. |
Publisher: | Elsevier (not including Cell Press) |
Date: | 2007 |
Volume: | 43 |
Number: | 56 |
Rights: | Copyright © 2010 Elsevier B.V. All rights reserved |
Divisions: | Schools > School of Science and Technology |
Record created by: | EPrints Services |
Date Added: | 09 Oct 2015 10:53 |
Last Modified: | 19 Oct 2015 14:38 |
URI: | https://irep.ntu.ac.uk/id/eprint/19729 |
Actions (login required)
Edit View |
Views
Views per month over past year
Downloads
Downloads per month over past year