Siqueira Santos, R.F., da Silva, N., Amstalden Junqueira, V.C., Kajsik, M., Forsythe, S. ORCID: 0000-0002-6709-0712 and Pereira, J.L., 2013. Screening forCronobacterSpecies in Powdered and Reconstituted Infant Formulas and from Equipment Used in Formula Preparation in Maternity Hospitals. Annals of Nutrition and Metabolism, 63 (1-2), pp. 62-68. ISSN 0250-6807
|
Text
PubSub4413_Forsythe.pdf - Post-print Download (193kB) | Preview |
Abstract
Background/Aims: Cronobacter spp. have been identified as being of considerable risk to neonates. The occurrence of organism in infant formulas is therefore of considerable interest. Methods: The occurrence of Cronobacter spp. in infant feeds (formulas and fortified cow’s milk) was determined using most probable number (MPN) analysis, and from formula preparation utensils. Ninety nine samples were analyzed, of which 42 were unopened cans of powdered infant formula (PIF), 25 reconstituted infant formulas in feeding bottles, 27 utensils used from the preparation of infant formula, and 5 samples of fortified cow’s milk. Presumptive Cronobacter spp. isolates were identified using the 7 allele multilocus sequence typing (MLST) scheme. Results: C. sakazakii, C. malonaticus and C. muytjensii were recovered from PIF. Although the incidence of Cronobacter in PIF was 29% (12/42), the level was low with an average of 0.54 MPN/100g. According to MLST profiling, C. sakazakii was the most frequently isolated Cronobacter species, and C. sakazakii ST4 (associated with neonatal meningitis) was recovered from 2/42 PIF samples at 0.51 and 0.92 MPN/100g. Conclusions: Cronobacter spp. can be isolated from PIF and therefore strict hygienic practices during PIF preparation are important to minimize neonate exposure and reduce the risk of severe infections.
Item Type: | Journal article | ||||
---|---|---|---|---|---|
Publication Title: | Annals of Nutrition and Metabolism | ||||
Creators: | Siqueira Santos, R.F., da Silva, N., Amstalden Junqueira, V.C., Kajsik, M., Forsythe, S. and Pereira, J.L. | ||||
Publisher: | S. Karger AG | ||||
Date: | 2013 | ||||
Volume: | 63 | ||||
Number: | 1-2 | ||||
ISSN: | 0250-6807 | ||||
Identifiers: |
|
||||
Divisions: | Schools > School of Science and Technology | ||||
Record created by: | Jill Tomkinson | ||||
Date Added: | 24 Feb 2016 16:51 | ||||
Last Modified: | 09 Jun 2017 13:59 | ||||
URI: | https://irep.ntu.ac.uk/id/eprint/27033 |
Actions (login required)
Edit View |
Views
Views per month over past year
Downloads
Downloads per month over past year