Mobility of lysozyme in poly(L-lysine)/hyaluronic acid multilayer films

Velk, N., Uhlig, K., Vikulina, A.S. ORCID: 0000-0001-9427-2055, Duschl, C. and Volodkin, D. ORCID: 0000-0001-7474-5329, 2016. Mobility of lysozyme in poly(L-lysine)/hyaluronic acid multilayer films. Colloids and Surfaces B: Biointerfaces, 147, pp. 343-350. ISSN 0927-7765

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The spatial and temporal control over presentation of protein-based biomolecules such as growth factors and hormones is crucial for in vitro applications to mimic the complex in vivo environment. We investigated the interaction of a model protein lysozyme (Lys) with poly(L-lysine)/hyaluronic acid (PLL/HA) multilayer films. We focused on Lys diffusion as well as adsorption and retention within the film as a function of the film deposition conditions and post-treatment. Additionally, an effect of Lys concentration on its mobility was probed. A combination of confocal fluorescence microscopy, fluorescence recovery after photobleaching, and microfluidics was employed for this investigation. Our main finding is that adsorption of PLL and HA after protein loading induces acceleration and reduction of Lys mobility, respectively. These results suggest that a charge balance in the film to a high extent governs the protein–film interaction. We believe that control over protein mobility is a key to reach the full potential of the PLL/HA films as reservoirs for biomolecules depending on the application demand.

Item Type: Journal article
Publication Title: Colloids and Surfaces B: Biointerfaces
Creators: Velk, N., Uhlig, K., Vikulina, A.S., Duschl, C. and Volodkin, D.
Publisher: Elsevier
Date: 1 November 2016
Volume: 147
ISSN: 0927-7765
S0927776516305574Publisher Item Identifier
Divisions: Schools > School of Science and Technology
Record created by: Linda Sullivan
Date Added: 10 Oct 2017 11:01
Last Modified: 30 Jul 2019 14:41

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