Austin, M., Elliott, L., Nicolaou, N., Grabowska, A. and Hulse, R.P. ORCID: 0000-0002-5193-9822, 2017. Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching. Oncotarget, 8 (44), pp. 76606-76621. ISSN 1949-2553
|
Text
PubSub9848_Hulse.pdf - Published version Download (4MB) | Preview |
Abstract
The tumour and neuron interaction has a significant impact upon disease progression and the patients quality of life. In breast cancer patients, it is known that there is an interaction between the tumour microenvironment and the sensory neurons to influence the progression of cancer as well as pain, though these mechanisms still need to be clearly defined. Here it is demonstrated that in a rodent orthotopic model of breast cancer (MDA MB 231) there was an increase in nerve fibre innervation into the tumour microenvironment (protein gene product 9.5), which were calcitonin gene related peptide positive C fibre nociceptors. In contrast, there was a reduction in myelinated nerve fibres (NF200). A sensory neuronal cell line was cultured in response to conditioned media from MDA MB231 and MCF7 as well as vascular endothelial growth factor-A (VEGF-A). All these experimental conditions induced sensory neuronal growth, with increased formation of collateral axonal branches. Furthermore, it was demonstrated that MDA MB231 and VEGF-A induced sensory neuronal sensitisation in response to capsaicin a TRPV1 agonist. MDA MB231 induced neuronal growth was suppressed by VEGFR2 inhibition (ZM323881 and neutralising antibody DC101), in addition both MDA MB231 and VEGF-A induced neurite growth was attenuated by the inhibition of ARP2/3 complex through co-treatment with CK666. This demonstrates that breast cancer can interact with the sensory nervous system to drive neuritogenesis through a VEGF-A/VEGFR2/ARP2/3 mediated pathway.
Item Type: | Journal article | ||||
---|---|---|---|---|---|
Publication Title: | Oncotarget | ||||
Creators: | Austin, M., Elliott, L., Nicolaou, N., Grabowska, A. and Hulse, R.P. | ||||
Publisher: | Impact Journals, LLC | ||||
Date: | 29 September 2017 | ||||
Volume: | 8 | ||||
Number: | 44 | ||||
ISSN: | 1949-2553 | ||||
Identifiers: |
|
||||
Rights: | Copyright: Austin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ||||
Divisions: | Schools > School of Science and Technology | ||||
Record created by: | Jill Tomkinson | ||||
Date Added: | 04 Jan 2018 15:32 | ||||
Last Modified: | 04 Jan 2018 15:32 | ||||
URI: | https://irep.ntu.ac.uk/id/eprint/32298 |
Actions (login required)
Edit View |
Views
Views per month over past year
Downloads
Downloads per month over past year