Antioxidative effects of flavonoids and their metabolites against hypoxia/reoxygenation-induced oxidative stress in a human first trimester trophoblast cell line

Ebegboni, VJ ORCID: https://orcid.org/0000-0002-1898-6555, Dickenson, JM ORCID: https://orcid.org/0000-0002-9683-969X and Sivasubramaniam, SD ORCID: https://orcid.org/0000-0001-7014-5000, 2019. Antioxidative effects of flavonoids and their metabolites against hypoxia/reoxygenation-induced oxidative stress in a human first trimester trophoblast cell line. Food Chemistry, 27, pp. 117-125. ISSN 0308-8146

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Abstract

This study aimed to investigate the cytoprotective effects of flavonoids, their metabolites alone or in combination against hypoxia/reoxygenation induced oxidative stress in the transformed human first trimester trophoblast cell line (HTR-8/SVneo).

Oxidative stress was achieved with hypoxia followed by reoxygenation and the following assays were performed: MTT, CellTox™ Green Cytotoxicity, CellTiter-Glo®, NADP/NADPH-Glo™, ROS-Glo™/H2O2, GSH/GSSG-Glo™ and Caspase-Glo® 3/7 assays.

HTR-8/SVneo cells, pre-treated for 24 h with flavonoids or their metabolites were protected significantly from oxidative stress. Flavonoids were associated with ROS modulation, reducing the generation of superoxide/hydrogen peroxide. The activities of caspases 3/7 were also significantly reduced significantly in HTR-8/SVneo cells pre-treated with flavonoids.

This study has shown for the first time that 24 h pre-treatment with flavonoids, their metabolites alone or in combination, protected against HR-induced oxidative stress in the trophoblast cell line. These data indicate that dietary flavonoids may be beneficial to placental health and invasion during early gestation.

Item Type:	Journal article
Publication Title:	Food Chemistry
Creators:	Ebegboni, V.J., Dickenson, J.M. and Sivasubramaniam, S.D.
Publisher:	Elsevier
Date:	30 January 2019
Volume:	27
ISSN:	0308-8146
Identifiers:	Number Type 10.1016/j.foodchem.2018.08.036 DOI S0308814618314377 Publisher Item Identifier
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	13 Aug 2018 11:37
Last Modified:	09 Aug 2019 03:00
URI:	https://irep.ntu.ac.uk/id/eprint/34323

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