Ebegboni, V.J. ORCID: 0000-0002-1898-6555, Dickenson, J.M. ORCID: 0000-0002-9683-969X and Sivasubramaniam, S.D. ORCID: 0000-0001-7014-5000, 2019. Antioxidative effects of flavonoids and their metabolites against hypoxia/reoxygenation-induced oxidative stress in a human first trimester trophoblast cell line. Food Chemistry, 27, pp. 117-125. ISSN 0308-8146
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Abstract
This study aimed to investigate the cytoprotective effects of flavonoids, their metabolites alone or in combination against hypoxia/reoxygenation induced oxidative stress in the transformed human first trimester trophoblast cell line (HTR-8/SVneo).
Oxidative stress was achieved with hypoxia followed by reoxygenation and the following assays were performed: MTT, CellTox™ Green Cytotoxicity, CellTiter-Glo®, NADP/NADPH-Glo™, ROS-Glo™/H2O2, GSH/GSSG-Glo™ and Caspase-Glo® 3/7 assays.
HTR-8/SVneo cells, pre-treated for 24 h with flavonoids or their metabolites were protected significantly from oxidative stress. Flavonoids were associated with ROS modulation, reducing the generation of superoxide/hydrogen peroxide. The activities of caspases 3/7 were also significantly reduced significantly in HTR-8/SVneo cells pre-treated with flavonoids.
This study has shown for the first time that 24 h pre-treatment with flavonoids, their metabolites alone or in combination, protected against HR-induced oxidative stress in the trophoblast cell line. These data indicate that dietary flavonoids may be beneficial to placental health and invasion during early gestation.
Item Type: | Journal article | ||||||
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Publication Title: | Food Chemistry | ||||||
Creators: | Ebegboni, V.J., Dickenson, J.M. and Sivasubramaniam, S.D. | ||||||
Publisher: | Elsevier | ||||||
Date: | 30 January 2019 | ||||||
Volume: | 27 | ||||||
ISSN: | 0308-8146 | ||||||
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Divisions: | Schools > School of Science and Technology | ||||||
Record created by: | Jonathan Gallacher | ||||||
Date Added: | 13 Aug 2018 11:37 | ||||||
Last Modified: | 09 Aug 2019 03:00 | ||||||
URI: | https://irep.ntu.ac.uk/id/eprint/34323 |
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