Metabolic reprogramming in nodules, roots and leaves of symbiotic soybean in response to iron deficiency

Chu, Q, Sha, Z, Maruyama, H, Yang, L, Pan, G ORCID: https://orcid.org/0000-0003-0920-3018, Xue, L and Watanabe, T, 2019. Metabolic reprogramming in nodules, roots and leaves of symbiotic soybean in response to iron deficiency. Plant, Cell & Environment, 42 (11), pp. 3027-3043. ISSN 0140-7791

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Abstract

To elucidate the mechanism of adaptation of leguminous plants to iron (Fe)‐deficient environment, comprehensive analyses of soybean (Glycine max) plants (sampled at anthesis) were conducted under Fe‐sufficient control and Fe‐deficient treatment using metabolomic and physiological approach. Our results show that soybeans grown under Fe‐deficient conditions showed lower nitrogen (N) fixation efficiency, however, ureides increased in different tissues, indicating potential N feedback inhibition. N assimilation was inhibited as observed in the repressed amino acids biosynthesis and reduced proteins in roots and nodules. In Fe‐deficient leaves, many amino acids increased, accompanied by the reduction of malate, fumarate, succinate and α‐ketoglutarate, which implies the N reprogramming was stimulated by the anaplerotic pathway. Accordingly, many organic acids increased in roots and nodules, however, enzymes involved in the related metabolic pathway (e.g., Krebs cycle) showed opposite activity between roots and nodules, indicative of different mechanisms. Sugars increased or maintained at constant level in different tissues under Fe deficiency, which probably relates to oxidative stress, cell wall damage and feedback regulation. Increased ascorbate, nicotinate, raffinose, galactinol and proline in different tissues possibly helped resist the oxidative stress induced by Fe deficiency. Overall, Fe deficiency induced the coordinated metabolic reprogramming in different tissues of symbiotic soybean plants.

Item Type:	Journal article
Publication Title:	Plant, Cell & Environment
Creators:	Chu, Q., Sha, Z., Maruyama, H., Yang, L., Pan, G., Xue, L. and Watanabe, T.
Publisher:	Wiley-Blackwell
Date:	November 2019
Volume:	42
Number:	11
ISSN:	0140-7791
Identifiers:	Number Type 10.1111/pce.13608 DOI 1072235 Other
Divisions:	Schools > School of Animal, Rural and Environmental Sciences
Record created by:	Jonathan Gallacher
Date Added:	11 Jul 2019 07:56
Last Modified:	09 Jul 2021 15:12
URI:	https://irep.ntu.ac.uk/id/eprint/37064

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