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Nikolopoulou, E., Papacleovoulou, G., Jean-Alphonse, F., Grimaldi, G., Parker, M.G., Hanyaloglu, A.C. and Christian, M. 
ORCID: 0000-0002-1616-4179,
2014.
Arachidonic acid-dependent gene regulation during preadipocyte differentiation controls adipocyte potential.
Journal of Lipid Research, 55 (12), pp. 2479-2490.
ISSN 0022-2275
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Abstract
Arachidonic acid (AA) is a major PUFA that has been implicated in the regulation of adipogenesis. We examined the effect of a short exposure to AA at different stages of 3T3-L1 adipocyte differentiation. AA caused the upregulation of fatty acid binding protein 4 (FABP4/aP2) following 24 h of differentiation. This was mediated by the prostaglandin F2α (PGF2α), as inhibition of cyclooxygenases or PGF2α receptor signaling counteracted the AA-mediated aP2 induction. In addition, calcium, protein kinase C, and ERK are all key elements of the pathway through which AA induces the expression of aP2. We also show that treatment with AA during the first 24 h of differentiation upregulates the expression of the transcription factor Fos-related antigen 1 (Fra-1) via the same pathway. Finally, treatment with AA for 24 h at the beginning of the adipocyte differentiation is sufficient to inhibit the late stages of adipogenesis through a Fra-1-dependent pathway, as Fra-1 knockdown rescued adipogenesis. Our data show that AA is able to program the differentiation potential of preadipocytes by regulating gene expression at the early stages of adipogenesis.
| Item Type: | Journal article | ||||
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| Publication Title: | Journal of Lipid Research | ||||
| Creators: | Nikolopoulou, E., Papacleovoulou, G., Jean-Alphonse, F., Grimaldi, G., Parker, M.G., Hanyaloglu, A.C. and Christian, M. | ||||
| Publisher: | American Society for Biochemistry and Molecular Biology | ||||
| Date: | December 2014 | ||||
| Volume: | 55 | ||||
| Number: | 12 | ||||
| ISSN: | 0022-2275 | ||||
| Identifiers: |
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| Rights: | Copyright © 2014 by the American Society for Biochemistry and Molecular Biology, Inc. | ||||
| Divisions: | Schools > School of Science and Technology | ||||
| Record created by: | Linda Sullivan | ||||
| Date Added: | 08 Aug 2019 13:25 | ||||
| Last Modified: | 08 Aug 2019 13:25 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/37196 |
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