Tools
Tools
Hammond, K.M., Sale, C. 
ORCID: 0000-0002-5816-4169, Fraser, W., Tang, J., Shepherd, S.O., Strauss, J.A., Close, G.L., Cocks, M., Louis, J., Pugh, J., Stewart, C., Sharples, A.P. and Morton, J.P.,
2019.
Post‐exercise carbohydrate and energy availability induce independent effects on skeletal muscle cell signalling and bone turnover: implications for training adaptation.
Journal of Physiology, 597 (18), pp. 4779-4796.
ISSN 0022-3751
|
Text
14521_Sale.pdf - Post-print Download (3MB) | Preview |
Abstract
We examined the effects of post-exercise carbohydrate (CHO) and energy availability (EA) on potent skeletal muscle cell signalling pathways (regulating mitochondrial biogenesis and lipid metabolism) and indicators of bone metabolism. In a repeated measures design, nine males completed a morning (AM) and afternoon (PM) high-intensity interval (HIT) (8 x 5-min at 85% VO2peak) running protocol (interspersed by 3.5 hours) under dietary conditions of 1) high CHO availability (HCHO: CHO ~12 g.kg-1 , EA~ 60 kcal.kg-1 FFM), 2) reduced CHO but high fat availability (LCHF: CHO ~3 g.kg-1 , EA~ 60 kcal.kg-1 FFM) or 3), reduced CHO and reduced energy availability (LCAL: CHO ~3 g.kg-1 , EA~ 20 kcal.kg-1 FFM). Muscle glycogen was reduced to ~200 mmol.kg-1 dw in all trials immediately post PM-HIT (P<0.01) and remained lower at 17-h (171, 194 and 316 mmol.kg-1 dw) post PM-HIT in LCHF and LCAL (P<0.001) compared to HCHO. Exercise induced comparable p38MAPK phosphorylation (P<0.05) immediately-post PM-HIT and similar mRNA expression (all P<0.05) of PGC-1α, p53 and CPT1 mRNA in HCHO, LCHF and LCAL. Post-exercise circulating βCTX was lower in HCHO (P<0.05) compared to LCHF and LCAL, whereas exercise-induced increases in IL-6 were larger in LCAL (P<0.05) compared to LCHF and HCHO. In conditions where glycogen concentration is maintained within 200-350 mmol.kg-1 dw, we conclude post-exercise CHO and energy restriction (i.e. <24 hours) does not potentiate cell signalling pathways that regulate hallmark adaptations associated with endurance training. In contrast, consuming CHO before, during and after HIT running attenuates bone resorption, effects that are independent of energy availability and circulating IL-6.
| Item Type: | Journal article | ||||||
|---|---|---|---|---|---|---|---|
| Publication Title: | Journal of Physiology | ||||||
| Creators: | Hammond, K.M., Sale, C., Fraser, W., Tang, J., Shepherd, S.O., Strauss, J.A., Close, G.L., Cocks, M., Louis, J., Pugh, J., Stewart, C., Sharples, A.P. and Morton, J.P. | ||||||
| Publisher: | Wiley on behalf of the Physiological Society | ||||||
| Date: | 15 September 2019 | ||||||
| Volume: | 597 | ||||||
| Number: | 18 | ||||||
| ISSN: | 0022-3751 | ||||||
| Identifiers: |
|
||||||
| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Jill Tomkinson | ||||||
| Date Added: | 15 Aug 2019 08:42 | ||||||
| Last Modified: | 31 May 2021 15:18 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/37324 |
Actions (login required)
 |
Edit View |
Views
Views per month over past year
Downloads
Downloads per month over past year
