An immunochemical analysis of monoamine oxidase in health and disease

Finch, C.C., 1999. An immunochemical analysis of monoamine oxidase in health and disease. PhD, Nottingham Trent University.

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Monoamine oxidase A (MAO-A) and monoamine oxidase B (MAO-B) molecular activities (catalytic activity per mole MAO protein) were measured in extracts of human placenta and platelets respectively. Specific competitive enzyme linked immunosorbant assay (ELISA) systems were used to measure MAO protein concentration and activity assays involved the use of phenylethylamine and dopamine (MAO-B) and 5- Hydroxytryptamine (5-HT, MAO-A) as substrates.

An assessment of the status of platelet MAO-B in de novo Parkinson's Disease (PD) has been conducted in both a Caucasian and Hong Kong Chinese population compared with age matched controls. In each population no significant differences in MAO status were observed between PD patients and controls irrespective of the substrate used (phenylethylamine or dopamine) and the sex of the subjects. Heterogeneity was observed in PD in both populations.

Platelet MAO-B concentration was significantly higher in Hong Kong Chinese patients compared with Caucasians in both the disease and control groups, suggesting a race difference.

MAO has been measured in human placenta using similar techniques. When placental extracts were analysed the competitive ELISAs estimated that MAO-A constitutes 90 % of the total MAO protein, MAO-B 10 %; the activity assays estimated 97 % MAO-A, 3 % MAO-B. However using immunohistochemical methods MAO-B is not detected in the placental tissue. These combined data suggest that the MAO-B detected in extracts is derived from blood elements (lymphocytes, platelets).

MAO-A has also been assessed in a preliminary study of pregnancy induced hypertension (pre-eclampsia) compared with normotensive controls. These preliminary results suggest that MAO-A molecular activity may be reduced in pre-eclampsia; this may contribute to the increase in maternal peripheral 5-HT observed in the disease.

Item Type: Thesis
Creators: Finch, C.C.
Date: 1999
ISBN: 9781369325416
Divisions: Schools > School of Science and Technology
Record created by: Linda Sullivan
Date Added: 28 Jun 2021 13:01
Last Modified: 13 Dec 2023 14:58

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