Yousefi, A. ORCID: 0009-0009-5446-4656, Ying, C. ORCID: 0000-0002-7279-1388, Parmenter, C.D.J., Assadipapari, M., Sanderson, G., Zheng, Z. ORCID: 0000-0002-9676-2856, Xu, L. ORCID: 0000-0001-9071-4311, Zargarbashi, S., Hickman, G.J. ORCID: 0000-0002-4632-9229, Cousins, R.B., Mellor, C.J., Mayer, M. and Rahmani, M. ORCID: 0000-0001-9268-4793, 2023. Optical monitoring of in situ iron loading into single, native ferritin proteins. Nano Letters, 23 (8), pp. 3251-3258. ISSN 1530-6984
Full text not available from this repository.Abstract
Ferritin is a protein that stores and releases iron to prevent diseases associated with iron dysregulation in plants, animals, and bacteria. The conversion between iron-loaded holo-ferritin and empty apo-ferritin is an important process for iron regulation. To date, studies of ferritin have used either ensemble measurements to quantify the characteristics of a large number of proteins or single-molecule approaches to interrogate labeled or modified proteins. Here we demonstrate the first real-time study of the dynamics of iron ion loading and biomineralization within a single, unlabeled ferritin protein. Using optical nanotweezers, we trapped single apo- and holo-ferritins indefinitely, distinguished one from the other, and monitored their structural dynamics in real time. The study presented here deepens the understanding of the iron uptake mechanism of ferritin proteins, which may lead to new therapeutics for iron-related diseases.
Item Type: | Journal article | ||||||
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Publication Title: | Nano Letters | ||||||
Creators: | Yousefi, A., Ying, C., Parmenter, C.D.J., Assadipapari, M., Sanderson, G., Zheng, Z., Xu, L., Zargarbashi, S., Hickman, G.J., Cousins, R.B., Mellor, C.J., Mayer, M. and Rahmani, M. | ||||||
Publisher: | American Chemical Society (ACS) | ||||||
Date: | 26 April 2023 | ||||||
Volume: | 23 | ||||||
Number: | 8 | ||||||
ISSN: | 1530-6984 | ||||||
Identifiers: |
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Rights: | Copyright © 2023 The Authors. Published by American Chemical Society. Open access using a Creative Commons Attribution licence 4.0 International (CC BY 4.0). | ||||||
Divisions: | Schools > School of Science and Technology | ||||||
Record created by: | Linda Sullivan | ||||||
Date Added: | 10 May 2023 09:42 | ||||||
Last Modified: | 08 Jul 2024 09:24 | ||||||
URI: | https://irep.ntu.ac.uk/id/eprint/48914 |
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