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Baba Ismail, Y.M., Reinwald, Y. 
ORCID: 0000-0001-6733-605X, Ferreira, A.M., Bretcanu, O., Dalgarno, K.W. and El Haj, A.J.,
2024.
Manufacturing of 3D-printed hybrid scaffolds with polyelectrolyte multilayer coating in static and dynamic culture conditions.
Materials, 17 (12): 2811.
ISSN 1996-1944
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1901956_Reinwald.pdf - Post-print Download (1MB) | Preview |
Abstract
Three-dimensional printing (3DP) has emerged as a promising method for creating intricate scaffold designs. This study assessed three 3DP scaffold designs fabricated using biodegradable poly(lactic) acid (PLA) through fused deposition modelling (FDM): mesh, two channels (2C), and four channels (4C). To address the limitations of PLA, such as hydrophobic properties and poor cell attachment, a post-fabrication modification technique employing Polyelectrolyte Multilayers (PEMs) coating was implemented. The scaffolds underwent aminolysis followed by coating with SiCHA nanopowders dispersed in hyaluronic acid and collagen type I, and finally crosslinked the outermost coated layers with EDC/NHS solution to complete the hybrid scaffold production. The study employed rotating wall vessels (RWVs) to investigate how simulating microgravity affects cell proliferation and differentiation. Human mesenchymal stem cells (hMSCs) cultured on these scaffolds using proliferation medium (PM) and osteogenic media (OM), subjected to static (TCP) and dynamic (RWVs) conditions for 21 days, revealed superior performance of 4C hybrid scaf-folds particularly in OM. Compared to commercial hydroxyapatite scaffolds, these hybrid scaf-folds demonstrated enhanced cell activity and survival. The pre-vascularization concept on 4C hybrid scaffolds showed the proliferation of both HUVECs and hMSCs throughout the scaffolds, with a positive expression of osteogenic and angiogenic markers at the early stages.
| Item Type: | Journal article | ||||||
|---|---|---|---|---|---|---|---|
| Publication Title: | Materials | ||||||
| Creators: | Baba Ismail, Y.M., Reinwald, Y., Ferreira, A.M., Bretcanu, O., Dalgarno, K.W. and El Haj, A.J. | ||||||
| Publisher: | MDPI | ||||||
| Date: | 8 June 2024 | ||||||
| Volume: | 17 | ||||||
| Number: | 12 | ||||||
| ISSN: | 1996-1944 | ||||||
| Identifiers: |
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| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Laura Ward | ||||||
| Date Added: | 07 Jun 2024 12:47 | ||||||
| Last Modified: | 04 Jul 2024 15:42 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/51540 |
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