Continuous, low latency estimation of the size and shape of single proteins from real-time nanopore data

Li, Y, Ying, C ORCID: https://orcid.org/0000-0002-7279-1388 and Mayer, M, 2026. Continuous, low latency estimation of the size and shape of single proteins from real-time nanopore data. Analytical Chemistry, 98 (1), pp. 224-234. ISSN 0003-2700

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Abstract

Existing approaches for nanopore sensing typically analyze resistive pulses from the translocation of individual proteins through the nanopore after completing the experiment. This approach foregoes instantaneous protein identification and precludes real-time experimental control. Here, we introduce a method for the analysis of real-time nanopore data capable of characterizing the size and approximate shape of proteins within a millisecond response time during data acquisition. The implemented real-time Two-Sliding Window (TSW) peak detection algorithm makes it possible, for the first time, to filter data, process baselines, and extract resistive pulse information during nanopore recordings using a stream of single data points. This approach achieves a computational throughput of 40 MB/s on a 1.8 GHz laptop CPU. We compared the accuracy of dwell time determination of the TSW algorithm with an established offline threshold searching (TS) algorithm, using simulated resistive pulses. The TSW algorithm accurately extracted events with dwell times greater than 1.5 times the reciprocal of the system’s cutoff frequency, 1.5 × (fc)−1. Moreover, we verify experimentally that integrating the TSW algorithm into the data acquisition process makes it possible to determine the approximate shape and volume of proteins within low-millisecond response times. Finally, by integrating a Naïve Bayes classifier, the system achieves real-time classification of protein mixtures, allowing for instant feedback control for manipulating single proteins during or immediately after translocation. This analysis also improves data storage efficiency during recordings with a high sampling rate.

Item Type:	Journal article
Publication Title:	Analytical Chemistry
Creators:	Li, Y., Ying, C. and Mayer, M.
Publisher:	American Chemical Society (ACS)
Date:	13 January 2026
Volume:	98
Number:	1
ISSN:	0003-2700
Identifiers:	Number Type 10.1021/acs.analchem.5c04044 DOI 2621458 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Laura Borcherds
Date Added:	28 Apr 2026 09:20
Last Modified:	28 Apr 2026 09:20
URI:	https://irep.ntu.ac.uk/id/eprint/55613

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