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Tran, B.Q., Barton, C., Feng, J., Sandjong, A., Yoon, S.H., Awasthi, S., Liang, T., Khan, M.M., Kilgour, D.P.A. 
ORCID: 0000-0002-3860-7532, Goodlett, D.R. and Goo, Y.A.,
2016.
Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.
Data in Brief, 6, pp. 68-76.
ISSN 2352-3409
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Abstract
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species. Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap. Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation. The data in this article is associated with the research article published in Journal of Proteomics (Tran et al., 2015).
| Item Type: | Journal article | ||||
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| Publication Title: | Data in Brief | ||||
| Creators: | Tran, B.Q., Barton, C., Feng, J., Sandjong, A., Yoon, S.H., Awasthi, S., Liang, T., Khan, M.M., Kilgour, D.P.A., Goodlett, D.R. and Goo, Y.A. | ||||
| Publisher: | Elsevier | ||||
| Date: | March 2016 | ||||
| Volume: | 6 | ||||
| ISSN: | 2352-3409 | ||||
| Identifiers: |
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| Divisions: | Schools > School of Science and Technology | ||||
| Record created by: | Jill Tomkinson | ||||
| Date Added: | 30 Jun 2016 15:21 | ||||
| Last Modified: | 09 Jun 2017 14:03 | ||||
| URI: | https://irep.ntu.ac.uk/id/eprint/28026 |
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