Tran, BQ, Barton, C, Feng, J, Sandjong, A, Yoon, SH, Awasthi, S, Liang, T, Khan, MM, Kilgour, DPA ORCID: https://orcid.org/0000-0002-3860-7532, Goodlett, DR and Goo, YA, 2016. Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry. Data in Brief, 6, pp. 68-76. ISSN 2352-3409
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Abstract
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species. Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap. Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation. The data in this article is associated with the research article published in Journal of Proteomics (Tran et al., 2015).
Item Type: | Journal article |
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Publication Title: | Data in Brief |
Creators: | Tran, B.Q., Barton, C., Feng, J., Sandjong, A., Yoon, S.H., Awasthi, S., Liang, T., Khan, M.M., Kilgour, D.P.A., Goodlett, D.R. and Goo, Y.A. |
Publisher: | Elsevier |
Date: | March 2016 |
Volume: | 6 |
ISSN: | 2352-3409 |
Identifiers: | Number Type 10.1016/j.dib.2015.11.031 DOI |
Divisions: | Schools > School of Science and Technology |
Record created by: | Jill Tomkinson |
Date Added: | 30 Jun 2016 15:21 |
Last Modified: | 09 Jun 2017 14:03 |
URI: | https://irep.ntu.ac.uk/id/eprint/28026 |
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