Modeling of Infrared–Visible Sum Frequency Generation Microscopy Images of a Giant Liposome

Volkov, V and Perry, CC ORCID: 0000-0003-1517-468X, 2016. Modeling of Infrared–Visible Sum Frequency Generation Microscopy Images of a Giant Liposome. Microscopy and Microanalysis. ISSN 1431-9276

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Abstract

The article explores the theory of Infrared-Visible Sum Frequency Generation microscopy of phospholipid envelopes with dimensions larger than the wavelength of the nonlinear emission. The main part of the study concerns derivation and accounting for the contributions of effective nonlinear responses specific to sites on the surfaces of a bilayer envelope and their dependence
on polarization condition and experimental geometry. The nonlinear responses of sites are mapped onto the image plane according to their emission directions and the numerical aperture of a sampling microscope objective. According to the simulation results, we discuss possible
approaches to characterize the shape of the envelope, to extract molecular hyperpolarizabilities, to anticipate possible heterogeneity in envelope composition and anisotropy of the environment proximal to the envelope. The modeling approach offers a promising analytic facility to assist connecting microscopy observations in engineered liposomes, cellular envelopes and sub-cellular
organelles of relatively large dimensions to molecular properties and hence to chemistry and structure down to available the spatial resolution.

Item Type: Journal article
Alternative Title: IR-VIS SFG liposome imaging
Publication Title: Microscopy and Microanalysis
Creators: Volkov, V. and Perry, C.C.
Publisher: Cambridge University Press for the Microscopy Society of America
Date: 27 October 2016
ISSN: 1431-9276
Identifiers:
NumberType
10.1017/S1431927616011752DOI
Divisions: Schools > School of Science and Technology
Depositing User: Jill Tomkinson
Date Added: 12 Dec 2016 12:13
Last Modified: 09 Jun 2017 14:09
URI: http://irep.ntu.ac.uk/id/eprint/29329

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