Volkov, V ORCID: https://orcid.org/0000-0003-2990-3580 and Perry, CC ORCID: https://orcid.org/0000-0003-1517-468X, 2016. Modeling of Infrared–Visible Sum Frequency Generation Microscopy Images of a Giant Liposome. Microscopy and Microanalysis. ISSN 1431-9276
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Abstract
The article explores the theory of Infrared-Visible Sum Frequency Generation microscopy of phospholipid envelopes with dimensions larger than the wavelength of the nonlinear emission. The main part of the study concerns derivation and accounting for the contributions of effective nonlinear responses specific to sites on the surfaces of a bilayer envelope and their dependence
on polarization condition and experimental geometry. The nonlinear responses of sites are mapped onto the image plane according to their emission directions and the numerical aperture of a sampling microscope objective. According to the simulation results, we discuss possible
approaches to characterize the shape of the envelope, to extract molecular hyperpolarizabilities, to anticipate possible heterogeneity in envelope composition and anisotropy of the environment proximal to the envelope. The modeling approach offers a promising analytic facility to assist connecting microscopy observations in engineered liposomes, cellular envelopes and sub-cellular
organelles of relatively large dimensions to molecular properties and hence to chemistry and structure down to available the spatial resolution.
Item Type: | Journal article |
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Alternative Title: | IR-VIS SFG liposome imaging |
Publication Title: | Microscopy and Microanalysis |
Creators: | Volkov, V. and Perry, C.C. |
Publisher: | Cambridge University Press for the Microscopy Society of America |
Date: | 27 October 2016 |
ISSN: | 1431-9276 |
Identifiers: | Number Type 10.1017/S1431927616011752 DOI |
Divisions: | Schools > School of Science and Technology |
Record created by: | Jill Tomkinson |
Date Added: | 12 Dec 2016 12:13 |
Last Modified: | 07 Feb 2022 14:54 |
URI: | https://irep.ntu.ac.uk/id/eprint/29329 |
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