Sub-lethal concentrations of CdCl2 disrupt cell migration and cytoskeletal proteins in cultured mouse TM4 Sertoli cells

Egbowon, B.F., Harris, W., Arnott, G., Mills, C.L. and Hargreaves, A.J. ORCID: 0000-0001-9754-5477, 2016. Sub-lethal concentrations of CdCl2 disrupt cell migration and cytoskeletal proteins in cultured mouse TM4 Sertoli cells. Toxicology in Vitro, 32, pp. 154-165. ISSN 0887-2333

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Abstract

The aims of this study were to examine the effects of CdCl2 on the viability, migration and cytoskeleton of cultured mouse TM4 Sertoli cells. Time- and concentration-dependent changes were exhibited by the cells but 1 µM CdCl2 was sub-cytotoxic at all time-points. Exposure to 1 and 12 µM CdCl2 for 4 h resulted in disruption of the leading edge, as determined by chemical staining. Cell migration was inhibited by both 1 and 12 µM CdCl2 in a scratch assay monitored by live cell imaging, although exposure to the higher concentration was associated with cell death. Western blotting and immunofluorescence staining indicated that CdCl2 caused a concentration dependent reduction in actin and tubulin levels. Exposure to Cd2+ also resulted in significant changes in the levels and/or phosphorylation status of the microtubule and microfilament destabilising proteins cofilin and stathmin, suggesting disruption of cytoskeletal dynamics. Given that 1-12 µM Cd2+ is attainable in vivo, our findings are consistent with the possibility that Cd2+ induced impairment of testicular development and reproductive health may involve a combination of reduced Sertoli cell migration and impaired Sertoli cell viability depending on the timing, level and duration of exposure.

Item Type: Journal article
Publication Title: Toxicology in Vitro
Creators: Egbowon, B.F., Harris, W., Arnott, G., Mills, C.L. and Hargreaves, A.J.
Publisher: Elsevier for the European Society of Toxicology in Vitro
Date: April 2016
Volume: 32
ISSN: 0887-2333
Identifiers:
NumberType
10.1016/j.tiv.2015.12.017DOI
Divisions: Schools > School of Science and Technology
Depositing User: Jill Tomkinson
Date Added: 21 Dec 2016 10:53
Last Modified: 09 Jun 2017 14:09
URI: http://irep.ntu.ac.uk/id/eprint/29480

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