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Egbowon, BF, Harris, W, Arnott, G, Mills, CL and Hargreaves, AJ 
ORCID: https://orcid.org/0000-0001-9754-5477,
2016.
Sub-lethal concentrations of CdCl2 disrupt cell migration and cytoskeletal proteins in cultured mouse TM4 Sertoli cells.
Toxicology in Vitro, 32, pp. 154-165.
ISSN 0887-2333
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Abstract
The aims of this study were to examine the effects of CdCl2 on the viability, migration and cytoskeleton of cultured mouse TM4 Sertoli cells. Time- and concentration-dependent changes were exhibited by the cells but 1 µM CdCl2 was sub-cytotoxic at all time-points. Exposure to 1 and 12 µM CdCl2 for 4 h resulted in disruption of the leading edge, as determined by chemical staining. Cell migration was inhibited by both 1 and 12 µM CdCl2 in a scratch assay monitored by live cell imaging, although exposure to the higher concentration was associated with cell death. Western blotting and immunofluorescence staining indicated that CdCl2 caused a concentration dependent reduction in actin and tubulin levels. Exposure to Cd2+ also resulted in significant changes in the levels and/or phosphorylation status of the microtubule and microfilament destabilising proteins cofilin and stathmin, suggesting disruption of cytoskeletal dynamics. Given that 1-12 µM Cd2+ is attainable in vivo, our findings are consistent with the possibility that Cd2+ induced impairment of testicular development and reproductive health may involve a combination of reduced Sertoli cell migration and impaired Sertoli cell viability depending on the timing, level and duration of exposure.
| Item Type: | Journal article |
|---|---|
| Publication Title: | Toxicology in Vitro |
| Creators: | Egbowon, B.F., Harris, W., Arnott, G., Mills, C.L. and Hargreaves, A.J. |
| Publisher: | Elsevier for the European Society of Toxicology in Vitro |
| Date: | April 2016 |
| Volume: | 32 |
| ISSN: | 0887-2333 |
| Identifiers: | Number Type 10.1016/j.tiv.2015.12.017 DOI |
| Divisions: | Schools > School of Science and Technology |
| Record created by: | Jill Tomkinson |
| Date Added: | 21 Dec 2016 10:53 |
| Last Modified: | 09 Jun 2017 14:09 |
| URI: | https://irep.ntu.ac.uk/id/eprint/29480 |
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