An acoustic on-chip goniometer for room temperature macromolecular crystallography

Burton, C.G., Axford, D., Edwards, A.M.J., Gildea, R.J., Morris, R.H. ORCID: 0000-0001-5511-3457, Newton, M.I. ORCID: 0000-0003-4231-1002, Orville, A.M., Prince, M., Topham, P. and Docker, P., 2017. An acoustic on-chip goniometer for room temperature macromolecular crystallography. Lab on a Chip. ISSN 1473-0197

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Abstract

This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

Item Type: Journal article
Publication Title: Lab on a Chip
Creators: Burton, C.G., Axford, D., Edwards, A.M.J., Gildea, R.J., Morris, R.H., Newton, M.I., Orville, A.M., Prince, M., Topham, P. and Docker, P.
Publisher: Royal Society of Chemistry
Date: 1 November 2017
ISSN: 1473-0197
Identifiers:
NumberType
10.1039/C7LC00812KDOI
Divisions: Schools > School of Science and Technology
Depositing User: Linda Sullivan
Date Added: 08 Nov 2017 16:38
Last Modified: 08 Nov 2017 16:38
URI: http://irep.ntu.ac.uk/id/eprint/32001

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