An acoustic on-chip goniometer for room temperature macromolecular crystallography

Burton, CG, Axford, D, Edwards, AMJ ORCID: https://orcid.org/0000-0003-3338-1287, Gildea, RJ, Morris, RH ORCID: https://orcid.org/0000-0001-5511-3457, Newton, MI ORCID: https://orcid.org/0000-0003-4231-1002, Orville, AM, Prince, M, Topham, P and Docker, P, 2017. An acoustic on-chip goniometer for room temperature macromolecular crystallography. Lab on a Chip, 17 (24), pp. 4225-4230. ISSN 1473-0197

Preview

Text PubSub9456_Newton.pdf - Post-print Download (1MB) | Preview

Abstract

This paper describes the design, development and succesful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for randomised in-fluid sample orientation and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facillitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

Item Type:	Journal article
Publication Title:	Lab on a Chip
Creators:	Burton, C.G., Axford, D., Edwards, A.M.J., Gildea, R.J., Morris, R.H., Newton, M.I., Orville, A.M., Prince, M., Topham, P. and Docker, P.
Publisher:	Royal Society of Chemistry
Date:	1 November 2017
Volume:	17
Number:	24
ISSN:	1473-0197
Identifiers:	Number Type 10.1039/C7LC00812K DOI
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	08 Nov 2017 16:38
Last Modified:	29 Oct 2020 16:48
URI:	https://irep.ntu.ac.uk/id/eprint/32001

Actions (login required)

Edit View

Statistics

Views

Downloads