Multi-fractional analysis of molecular diffusion in polymer multilayers by FRAP: a new simulation-based approach

Sustr, D., Hlaváček, A., Duschl, C. and Volodkin, D. ORCID: 0000-0001-7474-5329, 2018. Multi-fractional analysis of molecular diffusion in polymer multilayers by FRAP: a new simulation-based approach. The Journal of Physical Chemistry B, 122 (3), pp. 1323-1333. ISSN 1520-6106

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Abstract

Comprehensive analysis of the multifractional molecular diffusion provides a deeper understanding of the diffusion phenomenon in the fields of material science, molecular and cell biology, advanced biomaterials, etc. Fluorescence recovery after photobleaching (FRAP) is commonly employed to probe the molecular diffusion. Despite FRAP being a very popular method, it is not easy to assess multifractional molecular diffusion due to limited possibilities of approaches for analysis. Here we present a novel simulation-optimization-based approach (S-approach) that significantly broadens possibilities of the analysis. In the S-approach, possible fluorescence recovery scenarios are primarily simulated and afterward compared with a real measurement while optimizing parameters of a model until a sufficient match is achieved. This makes it possible to reveal multifractional molecular diffusion. Fluorescent latex particles of different size and fluorescein isothiocyanate in an aqueous medium were utilized as test systems. Finally, the S-approach has been used to evaluate diffusion of cytochrome c loaded into multilayers made of hyaluronan and polylysine. Software for evaluation of multifractional molecular diffusion by S-approach has been developed aiming to offer maximal versatility and user-friendly way for analysis.

Item Type: Journal article
Publication Title: The Journal of Physical Chemistry B
Creators: Sustr, D., Hlaváček, A., Duschl, C. and Volodkin, D.
Publisher: American Chemical Society
Date: 2018
Volume: 122
Number: 3
ISSN: 1520-6106
Identifiers:
NumberType
10.1021/acs.jpcb.7b11051DOI
Divisions: Schools > School of Science and Technology
Depositing User: Linda Sullivan
Date Added: 26 Feb 2018 12:38
Last Modified: 26 Feb 2018 12:38
URI: http://irep.ntu.ac.uk/id/eprint/32800

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