Fluorescently-tagged polyamines for the staining of siliceous materials

Annenkov, V.V., Danilovtseva, E.N., Pal'shin, V.A., Verkhozina, O.N., Shishlyannikova, T.A., Hickman, G.J. and Perry, C.C. ORCID: 0000-0003-1517-468X, 2018. Fluorescently-tagged polyamines for the staining of siliceous materials. Plant Physiology and Biochemistry, 125, pp. 205-211. ISSN 0981-9428

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Abstract

Siliceous frustules of diatom algae contain unique long-chain polyamines, including those having more than six nitrogen atoms. These polyamines participate in the formation of the siliceous frustules of the diatoms but their precise physiological role is not clear. The main hypotheses include formation of a polyamine and polyphosphate supramolecular matrix. We have synthesized novel fluorescent dyes from a synthetic oligomeric mixture of polyamines and the fluorophore 7-nitro-2,1,3-benzoxadiazole. The long polyamine chain ensures the high affinity of these dyes to silica, which allows their application in the staining of siliceous materials, such as valves of diatom algae and fossilized samples from sediments. The fluorescently stained diatom valves were found to be promising liquid flow tracers in hydrodynamic tests. Furthermore, complexation of the polyamine component of the dyes with carbonic polymeric acids results in changes to the visible spectrum of the fluorophore, which allows study of the stability of the complex vs the length of the polyamine chain. Using poly (vinyl phosphonic acid) as a model for phosphate functionality in silaffins (a potential matrix in the formation of biogenic silica) little complexation with the polyamine fluorophores was observed, bringing into question the role of a polyamine - polymeric phosphate matrix in biosilicification.

Item Type: Journal article
Publication Title: Plant Physiology and Biochemistry
Creators: Annenkov, V.V., Danilovtseva, E.N., Pal'shin, V.A., Verkhozina, O.N., Shishlyannikova, T.A., Hickman, G.J. and Perry, C.C.
Publisher: Elsevier Masson
Date: April 2018
Volume: 125
ISSN: 0981-9428
Identifiers:
NumberType
10.1016/j.plaphy.2018.02.014DOI
S0981942818300664Publisher Item Identifier
Divisions: Schools > School of Science and Technology
Depositing User: Jonathan Gallacher
Date Added: 16 Mar 2018 16:30
Last Modified: 16 Mar 2018 16:30
URI: http://irep.ntu.ac.uk/id/eprint/33014

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