Annenkov, VV, Danilovtseva, EN, Pal'shin, VA, Verkhozina, ON, Shishlyannikova, TA, Hickman, GJ ORCID: https://orcid.org/0000-0002-4632-9229 and Perry, CC ORCID: https://orcid.org/0000-0003-1517-468X, 2018. Fluorescently-tagged polyamines for the staining of siliceous materials. Plant Physiology and Biochemistry, 125, pp. 205-211. ISSN 0981-9428
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Abstract
Siliceous frustules of diatom algae contain unique long-chain polyamines, including those having more than six nitrogen atoms. These polyamines participate in the formation of the siliceous frustules of the diatoms but their precise physiological role is not clear. The main hypotheses include formation of a polyamine and polyphosphate supramolecular matrix. We have synthesized novel fluorescent dyes from a synthetic oligomeric mixture of polyamines and the fluorophore 7-nitro-2,1,3-benzoxadiazole. The long polyamine chain ensures the high affinity of these dyes to silica, which allows their application in the staining of siliceous materials, such as valves of diatom algae and fossilized samples from sediments. The fluorescently stained diatom valves were found to be promising liquid flow tracers in hydrodynamic tests. Furthermore, complexation of the polyamine component of the dyes with carbonic polymeric acids results in changes to the visible spectrum of the fluorophore, which allows study of the stability of the complex vs the length of the polyamine chain. Using poly (vinyl phosphonic acid) as a model for phosphate functionality in silaffins (a potential matrix in the formation of biogenic silica) little complexation with the polyamine fluorophores was observed, bringing into question the role of a polyamine - polymeric phosphate matrix in biosilicification.
Item Type: | Journal article |
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Publication Title: | Plant Physiology and Biochemistry |
Creators: | Annenkov, V.V., Danilovtseva, E.N., Pal'shin, V.A., Verkhozina, O.N., Shishlyannikova, T.A., Hickman, G.J. and Perry, C.C. |
Publisher: | Elsevier Masson |
Date: | April 2018 |
Volume: | 125 |
ISSN: | 0981-9428 |
Identifiers: | Number Type 10.1016/j.plaphy.2018.02.014 DOI S0981942818300664 Publisher Item Identifier |
Divisions: | Schools > School of Science and Technology |
Record created by: | Jonathan Gallacher |
Date Added: | 16 Mar 2018 16:30 |
Last Modified: | 06 Sep 2021 15:29 |
URI: | https://irep.ntu.ac.uk/id/eprint/33014 |
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