Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

Imrie, H. ORCID: 0000-0001-5214-3706 and Williams, D.J.L., 2019. Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity. BMC Veterinary Research, 15 (1): 45. ISSN 1746-6148

[img]
Preview
Text
13700_Imrie.pdf - Published version

Download (1MB) | Preview

Abstract

Background: Bacterial lipopolysaccharide and interferon-γ stimulation of rodent macrophages in vitro induces up-regulation of inducible nitric oxide synthase, whereas interleukin-4 stimulation results in increased activity of arginase-1. Thus different stimulants result in differing macrophage phenotypes, appropriate for responses to a range of pathogens. The current study was conducted in order to determine whether bovine macrophages derived from monocytes and spleen respond similarly.

Results: Lipopolysaccharide and interferon-γ did not induce detectable increases in nitric oxide production by bovine monocyte-derived or splenic macrophages in vitro. Similarly, interleukin-4 and interleukin-13 did not affect arginase activity. However, changes in transcription of genes coding for these products were detected.

Conclusion: Differences between macrophage activation patterns exist between cattle and other species and these differences may occur during the post-transcription phase.

Item Type: Journal article
Publication Title: BMC Veterinary Research
Creators: Imrie, H. and Williams, D.J.L.
Date: 2019
Volume: 15
Number: 1
ISSN: 1746-6148
Identifiers:
NumberType
10.1186/s12917-019-1785-0DOI
Rights: © the author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Divisions: Schools > School of Animal, Rural and Environmental Sciences
Depositing User: Jonathan Gallacher
Date Added: 04 Apr 2019 13:14
Last Modified: 04 Apr 2019 13:14
URI: http://irep.ntu.ac.uk/id/eprint/36180

Actions (login required)

Edit View Edit View

Views

Views per month over past year

Downloads

Downloads per month over past year