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Imrie, H 
ORCID: https://orcid.org/0000-0001-5214-3706 and Williams, DJL,
2019.
Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity.
BMC Veterinary Research, 15 (1): 45.
ISSN 1746-6148
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Abstract
Background: Bacterial lipopolysaccharide and interferon-γ stimulation of rodent macrophages in vitro induces up-regulation of inducible nitric oxide synthase, whereas interleukin-4 stimulation results in increased activity of arginase-1. Thus different stimulants result in differing macrophage phenotypes, appropriate for responses to a range of pathogens. The current study was conducted in order to determine whether bovine macrophages derived from monocytes and spleen respond similarly.
Results: Lipopolysaccharide and interferon-γ did not induce detectable increases in nitric oxide production by bovine monocyte-derived or splenic macrophages in vitro. Similarly, interleukin-4 and interleukin-13 did not affect arginase activity. However, changes in transcription of genes coding for these products were detected.
Conclusion: Differences between macrophage activation patterns exist between cattle and other species and these differences may occur during the post-transcription phase.
| Item Type: | Journal article |
|---|---|
| Publication Title: | BMC Veterinary Research |
| Creators: | Imrie, H. and Williams, D.J.L. |
| Date: | 2019 |
| Volume: | 15 |
| Number: | 1 |
| ISSN: | 1746-6148 |
| Identifiers: | Number Type 10.1186/s12917-019-1785-0 DOI |
| Rights: | © the author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| Divisions: | Schools > School of Animal, Rural and Environmental Sciences |
| Record created by: | Jonathan Gallacher |
| Date Added: | 04 Apr 2019 13:14 |
| Last Modified: | 04 Apr 2019 13:14 |
| URI: | https://irep.ntu.ac.uk/id/eprint/36180 |
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