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Brew, O., Nikolopoulou, E., Hughes, A., Christian, M. 
ORCID: 0000-0002-1616-4179, Lee, Y., Oduwole, O., Sullivan, M.H.F. and Woodman, A.,
2016.
Quality of placental RNA: effects of explant size and culture duration.
Placenta, 46, pp. 45-48.
ISSN 0143-4004
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Abstract
We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.
| Item Type: | Journal article | ||||||
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| Publication Title: | Placenta | ||||||
| Creators: | Brew, O., Nikolopoulou, E., Hughes, A., Christian, M., Lee, Y., Oduwole, O., Sullivan, M.H.F. and Woodman, A. | ||||||
| Publisher: | Elsevier | ||||||
| Date: | October 2016 | ||||||
| Volume: | 46 | ||||||
| ISSN: | 0143-4004 | ||||||
| Identifiers: |
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| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Linda Sullivan | ||||||
| Date Added: | 09 Aug 2019 09:51 | ||||||
| Last Modified: | 09 Aug 2019 09:51 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/37204 |
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