Quality of placental RNA: effects of explant size and culture duration

Brew, O, Nikolopoulou, E, Hughes, A, Christian, M ORCID: https://orcid.org/0000-0002-1616-4179, Lee, Y, Oduwole, O, Sullivan, MHF and Woodman, A, 2016. Quality of placental RNA: effects of explant size and culture duration. Placenta, 46, pp. 45-48. ISSN 0143-4004

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Abstract

We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.

Item Type:	Journal article
Publication Title:	Placenta
Creators:	Brew, O., Nikolopoulou, E., Hughes, A., Christian, M., Lee, Y., Oduwole, O., Sullivan, M.H.F. and Woodman, A.
Publisher:	Elsevier
Date:	October 2016
Volume:	46
ISSN:	0143-4004
Identifiers:	Number Type 10.1016/j.placenta.2016.08.083 DOI S0143400416304891 Publisher Item Identifier
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	09 Aug 2019 09:51
Last Modified:	09 Aug 2019 09:51
URI:	https://irep.ntu.ac.uk/id/eprint/37204

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