Quality of placental RNA: effects of explant size and culture duration

Brew, O., Nikolopoulou, E., Hughes, A., Christian, M. ORCID: 0000-0002-1616-4179, Lee, Y., Oduwole, O., Sullivan, M.H.F. and Woodman, A., 2016. Quality of placental RNA: effects of explant size and culture duration. Placenta, 46, pp. 45-48. ISSN 0143-4004

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Abstract

We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided.

Item Type: Journal article
Publication Title: Placenta
Creators: Brew, O., Nikolopoulou, E., Hughes, A., Christian, M., Lee, Y., Oduwole, O., Sullivan, M.H.F. and Woodman, A.
Publisher: Elsevier
Date: October 2016
Volume: 46
ISSN: 0143-4004
Identifiers:
NumberType
10.1016/j.placenta.2016.08.083DOI
S0143400416304891Publisher Item Identifier
Divisions: Schools > School of Science and Technology
Depositing User: Linda Sullivan
Date Added: 09 Aug 2019 09:51
Last Modified: 09 Aug 2019 09:51
URI: http://irep.ntu.ac.uk/id/eprint/37204

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