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Surani, A.A. 
ORCID: 0000-0002-5180-9075, Colombo, S.L. ORCID: 0000-0001-7360-986X, Barlow, G., Foulds, G.A. ORCID: 0000-0002-2053-7580 and Montiel-Duarte, C. ORCID: 0000-0002-9144-8809,
2021.
Optimizing cell synchronization using nocodazole or double thymidine block.
In: A.S. Coutts ORCID: 0000-0002-5005-1864 and L. Weston, eds.,
Cell cycle oscillators.
Methods in molecular biology
(2329).
New York: Humana.
ISBN 9781071615379
|
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1429011_Montiel_Duarte.pdf - Post-print Download (706kB) | Preview |
Abstract
Cell synchronisation is crucial when studying events that take place at specific points of the cell cycle. Several chemical agents can be used to achieve the cell culture synchronisation but not all type of cells respond equally to a given concentration of these drugs. Here we describe a simple optimisation method to select concentrations and timings for nocodazole or thymidine treatments using fluorescence staining. In addition, we provide detailed protocols to arrest an asynchronous culture of either suspension or adherent cells in G1/S or in G2/M.
| Item Type: | Chapter in book | ||||||
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| Creators: | Surani, A.A., Colombo, S.L., Barlow, G., Foulds, G.A. and Montiel-Duarte, C. | ||||||
| Publisher: | Humana | ||||||
| Place of Publication: | New York | ||||||
| Date: | 2021 | ||||||
| Number: | 2329 | ||||||
| ISBN: | 9781071615379 | ||||||
| Identifiers: |
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| Divisions: | Schools > School of Science and Technology | ||||||
| Record created by: | Linda Sullivan | ||||||
| Date Added: | 05 Jul 2021 13:07 | ||||||
| Last Modified: | 04 Jul 2022 15:30 | ||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/43312 |
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