Optimizing cell synchronization using nocodazole or double thymidine block

Surani, AA ORCID: https://orcid.org/0000-0002-5180-9075, Colombo, SL ORCID: https://orcid.org/0000-0001-7360-986X, Barlow, G, Foulds, GA ORCID: https://orcid.org/0000-0002-2053-7580 and Montiel-Duarte, C ORCID: https://orcid.org/0000-0002-9144-8809, 2021. Optimizing cell synchronization using nocodazole or double thymidine block. In: Coutts, AS ORCID: https://orcid.org/0000-0002-5005-1864 and Weston, L, eds., Cell cycle oscillators. Methods in molecular biology (2329). New York: Humana. ISBN 9781071615379

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Abstract

Cell synchronisation is crucial when studying events that take place at specific points of the cell cycle. Several chemical agents can be used to achieve the cell culture synchronisation but not all type of cells respond equally to a given concentration of these drugs. Here we describe a simple optimisation method to select concentrations and timings for nocodazole or thymidine treatments using fluorescence staining. In addition, we provide detailed protocols to arrest an asynchronous culture of either suspension or adherent cells in G1/S or in G2/M.

Item Type:	Chapter in book
Creators:	Surani, A.A., Colombo, S.L., Barlow, G., Foulds, G.A. and Montiel-Duarte, C.
Publisher:	Humana
Place of Publication:	New York
Date:	2021
Number:	2329
ISBN:	9781071615379
Identifiers:	Number Type 10.1007/978-1-0716-1538-6_9 DOI 1429011 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Linda Sullivan
Date Added:	05 Jul 2021 13:07
Last Modified:	04 Jul 2022 15:30
URI:	https://irep.ntu.ac.uk/id/eprint/43312

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