Retinal pigment epithelial cells can be cultured on fluocinolone acetonide treated nanofibrous scaffold

Egbowon, B.F. ORCID: 0000-0002-7397-7491, Fornari, E., Pally, J.M., Hargreaves, A.J. ORCID: 0000-0001-9754-5477, Stevens, B., McGinnity, T.M. and Pierscionek, B.K., 2023. Retinal pigment epithelial cells can be cultured on fluocinolone acetonide treated nanofibrous scaffold. Materials and Design, 232: 112152. ISSN 0264-1275

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Abstract

Engineered tissue currently lacks requisite capacity to sustain cell viability and functionality. Here we demonstrate that human RPE cell lines (ARPE-19) can be cultured on ultrathin suspended electrospun nanofibre scaffolds (ENS) composed of hydrophobic polymer polyacrylonitrile (PAN) and a water-soluble aliphatic diamine, without (untreated) or with (treated) fluocinolone acetonide (FA). Cells survived and retained their characteristic morphology for up to 150 days with FA-treated ENS and manifested a morphological epithelial phenotype with expression of biomarkers critical for maintaining retinal physiological characteristics. This novel technique for producing culture substrates provides suitable hydrophilicity and a protective environment for prolonged RPE culture and has immense potential for subretinal transplantation. The findings indicate that FA-treated ENS is an excellent matrix for retaining the differentiated and epithelial phenotype.

Item Type:	Journal article
Publication Title:	Materials and Design
Creators:	Egbowon, B.F., Fornari, E., Pally, J.M., Hargreaves, A.J., Stevens, B., McGinnity, T.M. and Pierscionek, B.K.
Publisher:	Elsevier
Date:	August 2023
Volume:	232
ISSN:	0264-1275
Identifiers:	Number Type 10.1016/j.matdes.2023.112152 DOI 1782934 Other
Divisions:	Schools > School of Science and Technology
Record created by:	Jonathan Gallacher
Date Added:	20 Jul 2023 09:28
Last Modified:	20 Jul 2023 09:28
URI:	https://irep.ntu.ac.uk/id/eprint/49404

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