Retinal pigment epithelial cells can be cultured on fluocinolone acetonide treated nanofibrous scaffold

Egbowon, BF ORCID logoORCID: https://orcid.org/0000-0002-7397-7491, Fornari, E, Pally, JM, Hargreaves, AJ ORCID logoORCID: https://orcid.org/0000-0001-9754-5477, Stevens, B, McGinnity, TM and Pierscionek, BK, 2023. Retinal pigment epithelial cells can be cultured on fluocinolone acetonide treated nanofibrous scaffold. Materials and Design, 232: 112152. ISSN 0264-1275

Full text not available from this repository.

Abstract

Engineered tissue currently lacks requisite capacity to sustain cell viability and functionality. Here we demonstrate that human RPE cell lines (ARPE-19) can be cultured on ultrathin suspended electrospun nanofibre scaffolds (ENS) composed of hydrophobic polymer polyacrylonitrile (PAN) and a water-soluble aliphatic diamine, without (untreated) or with (treated) fluocinolone acetonide (FA). Cells survived and retained their characteristic morphology for up to 150 days with FA-treated ENS and manifested a morphological epithelial phenotype with expression of biomarkers critical for maintaining retinal physiological characteristics. This novel technique for producing culture substrates provides suitable hydrophilicity and a protective environment for prolonged RPE culture and has immense potential for subretinal transplantation. The findings indicate that FA-treated ENS is an excellent matrix for retaining the differentiated and epithelial phenotype.

Item Type: Journal article
Publication Title: Materials and Design
Creators: Egbowon, B.F., Fornari, E., Pally, J.M., Hargreaves, A.J., Stevens, B., McGinnity, T.M. and Pierscionek, B.K.
Publisher: Elsevier
Date: August 2023
Volume: 232
ISSN: 0264-1275
Identifiers:
Number
Type
10.1016/j.matdes.2023.112152
DOI
1782934
Other
Divisions: Schools > School of Science and Technology
Record created by: Jonathan Gallacher
Date Added: 20 Jul 2023 09:28
Last Modified: 20 Jul 2023 09:28
URI: https://irep.ntu.ac.uk/id/eprint/49404

Actions (login required)

Edit View Edit View

Statistics

Views

Views per month over past year

Downloads

Downloads per month over past year