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Scott, R. 
ORCID: 0000-0001-7642-545X, Varley, I. ORCID: 0000-0002-3607-8921, Sale, C., Tarum, J. ORCID: 0009-0007-1690-7191, James, R. ORCID: 0000-0002-7119-3159, Barnett, C.T. ORCID: 0000-0001-6898-9095 and Santos, L. ORCID: 0000-0002-1915-6780,
2024.
Intermittent tensile strain induces an increased response in bone formation markers compared to continuous load in mouse pre-osteoblasts when loading magnitude is matched.
Journal of the Mechanical Behavior of Biomedical Materials, 159: 106683.
ISSN 1751-6161
Abstract
Intermittent and continuous mechanical loads are known to influence osteogenic activity. The present study examines the effects of matched intermittent and continuous load in vitro on bone formation markers. MC3T3 (mouse pre-osteoblasts) were cultured and placed in a bioreactor to undergo continuous, intermittent, or unloading for 1, 3 and 12 days. Loading conditions were matched for magnitude, duration and frequency. Each time point was analysed for alkaline phosphatase (ALP) activity, procollagen 1 N-terminal propeptide (PINP) and alizarin red staining (ARS). Intermittent load caused an increase in ALP activity across all time points compared to continuous loading (↑30%–59%) and unloaded conditions (↑70%–90%). PINP concentrations from intermittent load were lower than continuous load (↓112%) on day 3. However, no differences were observed in PINP concentrations between loading conditions at other time points. No differences were observed for ARS between loading conditions. Intermittent load caused an increase in bone formation marker ALP, but not PINP, when compared to continuous loading and unloaded conditions. These findings further our knowledge in bone formation response and provide additional tools for the analysis of osteogenesis in vitro.
| Item Type: | Journal article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Publication Title: | Journal of the Mechanical Behavior of Biomedical Materials | ||||||||
| Creators: | Scott, R., Varley, I., Sale, C., Tarum, J., James, R., Barnett, C.T. and Santos, L. | ||||||||
| Publisher: | Elsevier | ||||||||
| Date: | November 2024 | ||||||||
| Volume: | 159 | ||||||||
| ISSN: | 1751-6161 | ||||||||
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| Rights: | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | ||||||||
| Divisions: | Schools > School of Science and Technology | ||||||||
| Record created by: | Jonathan Gallacher | ||||||||
| Date Added: | 28 Aug 2024 09:42 | ||||||||
| Last Modified: | 28 Aug 2024 09:42 | ||||||||
| URI: | https://irep.ntu.ac.uk/id/eprint/52110 |
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